Expedited Assessment of Terrestrial Arthropod Diversity by Coupling Malaise Traps with DNA Barcoding

deWaard, J. R.; Levesque-Beaudin, V.; deWaard, S. L.; Ivanova, N. V.; McKeown, J. T. A.; Miskie, R.; Naik, S.; Perez, K.; Ratnasingham, S.; Sobel, C. N.; Sones, J. E.; Steinke, C.; Telfer, A. C.; Young, A. D.; Young, M. R.; Zakharov, E. V.; Hebert, P. D. N.

doi:10.1101/192732

bioRxiv · 10.1101/192732

Expedited Assessment of Terrestrial Arthropod Diversity by Coupling Malaise Traps with DNA Barcoding

deWaard, J. R.·Levesque-Beaudin, V.·deWaard, S. L.·Ivanova, N. V.·McKeown, J. T. A.·Miskie, R.·Naik, S.·Perez, K.·Ratnasingham, S.·Sobel, C. N.·Sones, J. E.·Steinke, C.·Telfer, A. C.·Young, A. D.·Young, M. R.·Zakharov, E. V.·Hebert, P. D. N.

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Abstract

O_LIMonitoring changes in terrestrial arthropod communities over space and time requires a dramatic increase in the speed and accuracy of processing samples that cannot be achieved with morphological approaches.\nC_LIO_LIThe combination of DNA barcoding and Malaise traps allows expedited, comprehensive inventories of species abundance whose cost will rapidly decline as high-throughput sequencing technologies advance.\nC_LIO_LIAside from detailing protocols from specimen sorting to data release, this paper describes their use in a survey of arthropod diversity in a national park that examined 20,000 specimens representing 2200 species.\nC_LIO_LIThese protocols can support arthropod monitoring programs at regional, national, and continental scales.\nC_LI

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deWaard, J. R., Levesque-Beaudin, V., deWaard, S. L., Ivanova, N. V., McKeown, J. T. A., Miskie, R., Naik, S., Perez, K., Ratnasingham, S., Sobel, C. N., Sones, J. E., Steinke, C., Telfer, A. C., Young, A. D., Young, M. R., Zakharov, E. V., Hebert, P. D. N.. 2017-09-25. Expedited Assessment of Terrestrial Arthropod Diversity by Coupling Malaise Traps with DNA Barcoding. https://doi.org/10.1101/192732

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