Ultra-fast super-resolution imaging of biomolecular mobility in tissues

Miller, H.; Cosgrove, J.; Wollman, A. J. M.; O'Toole, P. J.; Coles, M.; Leake, M. C.

doi:10.1101/179747

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bioRxiv · 10.1101/179747

Ultra-fast super-resolution imaging of biomolecular mobility in tissues

Miller, H.·Cosgrove, J.·Wollman, A. J. M.·O'Toole, P. J.·Coles, M.·Leake, M. C.

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Abstract

Super-resolution techniques have addressed many biological questions, yet molecular quantification at rapid timescales in live tissues remains challenging. We developed a light microscopy system capable of sub-millisecond sampling to characterize molecular diffusion in heterogeneous aqueous environments comparable to interstitial regions between cells in tissues. We demonstrate our technique with super-resolution tracking of fluorescently labelled chemokine molecules in a collagen matrix and ex vivo lymph node tissue sections, outperforming competing methods.

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Miller, H., Cosgrove, J., Wollman, A. J. M., O'Toole, P. J., Coles, M., Leake, M. C.. 2017-08-23. Ultra-fast super-resolution imaging of biomolecular mobility in tissues. https://doi.org/10.1101/179747

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