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bioRxiv · 10.64898/2026.10.25.761624

Correlation-resolved sum-frequency generation microscopy of biological tissue with bright twin beams

Chen, T.-H.·Adachi, M.·Hu, Z.·Masaki, M.·Nikaido, Y.·Suzuki, R.·Nuriya, M.·Eto, Y.·Kano, H.
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Abstract

Sum-frequency generation (SFG) driven by bright twin beams generated through high-gain parametric down-conversion comprises a coherent component arising from correlated mode pairs and an incoherent component arising from interactions between uncorrelated modes. Modal correlations can enhance coherent SFG in anisotropic nonlinear media, for example by extending the effective interaction length. Whether such correlations can likewise benefit image formation in heterogeneous biological tissue remains unknown. Here we address this question by performing SFG imaging of biological tissue using bright twin beams as the excitation source. Spectral decomposition of the SFG signal separates its coherent and incoherent components, allowing us to reconstruct co-registered images and examine how twin-beam correlations influence image formation. In mouse skeletal muscle, the coherent-component image revealed sarcomere periodicity with a Bragg-spot-to-background ratio of approximately 310, compared with 7.5 in the incoherent-component image. In fresh human dermis, the coherent-component image showed greater Fourier power than the incoherent-component image in the 0.30-0.50 \mu m^{-1} band and a greater fractional autocovariance decay at short spatial lags. These results demonstrate that twin-beam correlations can enhance contrast and detectability at selected spatial frequencies in heterogeneous tissue. Correlation-resolved SFG microscopy thus offers a route for exploiting bright quantum-light sources in nonlinear optical bioimaging.

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Chen, T.-H., Adachi, M., Hu, Z., Masaki, M., Nikaido, Y., Suzuki, R., Nuriya, M., Eto, Y., Kano, H.. 2026-10-30. Correlation-resolved sum-frequency generation microscopy of biological tissue with bright twin beams. https://doi.org/10.64898/2026.10.25.761624

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