Biology subjects

Tavares, A. P.

Publications and source records attributed to Tavares, A. P..

2 recordsLinked to original sources

bioRxiv2023

The Striatal Heterodimers A2AR-D2Rs Are Linked To Ergogenic Effects Of Caffeine

Adenosine 2A receptors (A2ARs) are abundant in the striatum, and they are found co-localized with dopamine D2 receptors (D2R) and in the heterodimer form A2AR-D2R. Previous we demonstrated that caffeine delays central fatigue through adenosine 2A receptors (A2ARs) antagonism, striatal neuroplasticity and mitochondrial metabolism enhancement. In this study we demonstrate the participation of striatal A2AR-D2R in the ergogenic outcomes. Two-hundred swiss adult mice were treated systemically (i.p.) and centrally (striatum-st.) with caffeine, SCH58261 (A2AR antagonist), DPCPX (A1R antagonist) and haloperidol (D2R antagonist), and performed the open field test, treadmill incremental exercise and grip strength meter test We observed that A2AR-D2R antagonic effects are linked to motor control (p < 0.05) and enhancement of power (p < 0.05). However, the role of A2AR-D2R in the mechanism of strength generation seems to differ from motor control.

neuroscience↗

bioRxiv2022

The striatum drives the ergogenic effects of caffeine

Caffeine is one of the main ergogenic resources used in exercise and sports. Previously, we reported the ergogenic mechanism of caffeine through neuronal A2AR antagonism in the central nervous system [1]. We now demonstrate that the striatum rules the ergogenic effects of caffeine through neuroplasticity changes. Thirty-four Swiss (8-10 weeks, 47 {+/-} 1.5 g) and twenty-four C57BL/6J (8-10 weeks, 23.9 {+/-} 0.4 g) adult male mice were studied behaviorly and electrophysiologically using caffeine and energy metabolism was studied in SH-SY5Y cells. Systemic (15 mg/kg, i.p.) or striatal (bilateral, 15 g) caffeine was psychostimulant in the open field (p < 0.05) and increased grip efficiency (p < 0.05). Caffeine also shifted long-term depression (LTD) to potentiation (LTP) in striatal slices and increased the mitochondrial mass (p < 0.05) and membrane potential (p < 0.05) in SH-SY5Y dopaminergic cells. Our results demonstrate the role of the striatum in the ergogenic effects of caffeine, with changes in neuroplasticity and mitochondrial metabolism.

neuroscience↗