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Stagg, C.

Publications and source records attributed to Stagg, C..

2 recordsLinked to original sources
bioRxiv2026

Driving theta-gamma oscillations modulates extrasynaptic GABAergic tone: a tACS-TMS study

BACKGROUNDTheta-gamma phase-amplitude coupled ({theta}{gamma}-PAC) oscillations in primary motor cortex (M1) have been shown to support motor skill acquisition. Past research has shown that driving gamma activity at the theta peak (TGP), but not the theta trough (TGT) using transcranial alternating current stimulation (tACS) enhances motor learning (Akkad et al., 2021). However, the neurophysiological mechanisms underlying this phase-specific effect remain unclear. METHODSIn a double-blind, sham-controlled, cross-over study, twenty-two healthy participants received 20 minutes of 75Hz/6Hz TGP-tACS, TGT-tACS, or sham stimulation over M1. We used paired-pulse transcranial magnetic stimulation (TMS) to assess GABAergic and NMDAR-mediated activity before, during, and after tACS. Outcome measures included short-interval intracortical inhibition at 1ms (SICI1ms; extrasynaptic GABAergic tone) and 2.5ms (SICI2.5ms; synaptic GABAA activity), intracortical facilitation at 12ms (ICF12ms; NMDAR activity), and motor evoked potential (MEP) amplitude (corticospinal excitability). RESULTSTGP-tACS selectively decreased SICI1ms, a putative marker of extrasynaptic GABAergic tone (main effect of Stimulation: p=.021), with significant differences between TGP and TGT during late stimulation (p=.047). No significant effects were observed on corticospinal excitability, synaptic GABAergic activity (SICI2.5ms), or NMDAR signalling (ICF12ms). CONCLUSIONSDriving theta-gamma oscillations at the theta peak using tACS specifically modulates extrasynaptic GABAergic tone in M1 without affecting corticospinal excitability or synaptic inhibition. Given that reductions in GABAergic signalling supports motor learning, these findings provide a neurophysiological mechanism for the phase-specific behavioural effects of {theta}{gamma}-PAC tACS and suggest a potential therapeutic approach for facilitating motor recovery after stroke. HighlightsO_LITheta-gamma peak tACS selectively reduces extrasynaptic GABA in human motor cortex C_LIO_LIOnly gamma at the peak, not trough, of theta stimulation modulates GABA C_LIO_LINo effects on corticospinal excitability, synaptic GABA, or NMDAR signalling C_LIO_LItACS-TMS reveals mechanism for phase-dependent motor learning effects C_LI

neuroscience↗
bioRxiv2020

GABA relates to functional connectivity changes and retention in visuomotor adaptation

Motor adaptation is fundamental to maintaining accurate movements under changing conditions, but the neurochemical basis of human motor adaptation is not well understood. Here, we used an ultra-high-field MR multimodal acquisition to address the hypothesis that M1 GABA and M1-Cerebellar functional connectivity would relate to retention of adaptation, but not acquisition of adaptation. As such, we demonstrate higher baseline M1 [GABA] relates to greater retention but does not relate to adaptation-acquisition. This relationship is mediated by change in M1-Cerebellar functional connectivity: higher M1 [GABA] relates to a decreased M1-Cerebellar connectivity, resulting in greater retention. These findings showed anatomical, neurochemical and behavioural specificity: As expected, no relationship was found between retention and a control metabolite, retention and connectivity change between control regions and between M1 [GABA] and behaviour in a control condition. The implication of a mechanistic link from neurochemistry to retention advances our understanding of population variability in retention and provides a step towards therapeutic interventions to restore motor abilities.

neuroscience↗