Biology subjects

Schueth, A.

Publications and source records attributed to Schueth, A..

5 recordsLinked to original sources

bioRxiv2026

Light-sheet fluorescence microscopy of optically cleared human head-and-neck organs: three-dimensional tissue assessment and histological correlation

Conventional histology provides detailed cellular information but only a partial view of the three-dimensional (3D) organisation of head-and-neck tissues. We investigated whether optical tissue clearing combined with light-sheet fluorescence microscopy (LSFM) could visualise the microanatomy of large head-and-neck specimens and relate volumetric features to conventional histology. Non-neoplastic human palatine tonsil, cervical lymph node, tongue and parotid gland specimens were cleared with a solvent-based protocol, labelled with small-molecule dyes and imaged by LSFM, with haematoxylin and eosin (H&E) histology as a morphological reference. LSFM visualised the characteristic architecture of each tissue, including lymphoid follicles and crypt epithelium in tonsil, cortex, paracortex and follicles in lymph node, epithelium and intrinsic muscle fibres in tongue, and lobules, ducts and adipocytes in parotid. Following structures through successive optical sections revealed their continuity and branching over long distances, information not available from single sections. Tongue epithelial measurements were comparable with H&E, and segmentation enabled volumetric quantification of structures such as the tongue epithelium and parotid adipocytes. Optical clearing and LSFM thus provide a feasible single workflow for volumetric investigation of human head-and-neck microanatomy. These data establish a 3D morphological reference with potential applications in cancer research and staging, computational image analysis and anatomical education, to be further developed through larger donor cohorts and specific labelling.

pathology↗

bioRxiv2019

Monte Carlo Simulation of Diffusion MRI in geometries constructed from two-photon microscopy of cortical gray matter

PurposeNeurodegenerative diseases such as Alzheimers disease cause changes and disruption to cortical microstructure and architecture. Diffusion MRI (dMRI) could potentially be sensitive to such changes. There is a growing interest in modeling of human cortical areas using a combination of quantitative MRI and 3D microscopy. The purpose of this study was to quantitatively characterize the cytoarchitecture of human cortical tissue from 3D fluorescence microscopy to simulate diffusion MRI (dMRI) signal in the cortex to better understand its diffusion signal characteristics.\n\nMethodsDiffusion of water molecules and dMRI signal were simulated by an indirect geometry based method and a direct voxel based method in microstructural details extracted from microscopy of cortex. Additionally, residence times of diffusing spins inside voxel volumes were considered to set effective resolution limits. Mean diffusivity (MD) and kurtosis (MK) were calculated for variable cell and neurite densities, sizes and diffusion times under realistic values for permeability and free diffusion.\n\nResultsBoth simulation methods could efficiently and accurately simulate dMRI signals with fractional anisotropy, diffusion coefficient and kurtosis in agreement with previous reports. Simulated MD and MK showed changes with increasing diffusion times specific to cortical cell density and sizes, with MK showing the highest sensitivity. Intra-voxel residence times with increasing diffusion times showed that the effective dMRI resolution approaches the thickness of cortical layers.\n\nConclusionsMonte Carlo simulations based on 3D microscopy data enable estimating changes in MD and MK over diffusion times and are sensitive to cortical cytoarchitecture and its possible changes in neurodegenerative disease. When considering layer-specific cortical dMRI, effective resolution due to residence times is an important concern.

biophysics↗

bioRxiv2019

The mesoSPIM initiative: open-source light-sheet mesoscopes for imaging in cleared tissue

Over the course of the past decade, tissue clearing methods have reached a high level of sophistication with a wide variety of approaches now available1. To image large cleared samples, light-sheet microscopes have proven to be ideal due to their excellent optical sectioning capability in transparent tissue2. Such instruments have recently seen extensive technological and commercial development. However, despite this progress, the community is lacking instruments capable of exploring large samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy (mesoSPIM) initiative, an open-hardware project that provides researchers with instructions and software to easily build and operate light-sheet microscopes for centimeter-sized cleared samples (http://www.mesospim.org).\n\nA wide range of commercial light-sheet microscopes have ...

neuroscience↗

bioRxiv2018

High-resolution bladder morphology in the intact mouse bladder: an intravital two-photon study

We developed a platform for intravital bladder two-photon microscopy (TPM) in healthy wild-type mice. It was our aim to make intravital bladder TPM simple and reproducible for researchers with access to TPM. In this study, the intact murine bladder was examined by means of autofluorescence (AF), second-harmonic generation (SHG), and different i.v. injected fluorescent dyes. All bladder layers with containing structures, such as cells, nerves, and vessels were detected in different colour-coded spectral channels, and shown in high-resolution images and real-time movies. The presented method opens up avenues for many future studies and applications, such as to reveal mechanisms and physiology in the natural state of the bladder. Researcher can use intravital bladder TPM to investigate events, such as migration and metabolism of (inflammatory) cells, nanoparticle uptake, or micro contractions in response to stimuli.

cell biology↗

bioRxiv2018

Scalable cytoarchitectonic characterization of large intact human neocortex samples

We describe MASH (Multiscale Architectonic Staining of Human cortex): a simple, fast and low-cost cytoarchitectonic labeling and optical clearing approach for human cortex samples, which can be applied to large formalin fixed adult brain samples. A suite of small-molecule fluorescent nuclear and cytoplasmic dyes in combination with new refractive index matching solutions allows deep volume imaging. This enables highly scalable human neocortical cytoarchitecture characterization with a large 3D scope.

neuroscience↗