Biology subjects

Grassin, Q.

Publications and source records attributed to Grassin, Q..

3 recordsLinked to original sources

bioRxiv2024

Antiviral activity of Tecovirimat against Mpox virus clades 1a, 1b, 2a and 2b

The recent Mpox virus (MPXV) outbreak was caused by a novel and more pathogenic clade 1b virus. We compared the antiviral efficacy of Tecovirimat in cell culture, against the two ancestral clades 1a and 2a, the clade 2b that circulated in 2022, and the recent clade 1b virus. We report that Tecovirimat similarly inhibits the replication of all four MPXV clades, at nanomolar concentrations (nM). Our results suggest that Tecovirimat remains a therapeutic option against the latest clade 1b virus.

microbiology↗

bioRxiv2022

Heat inactivation of the Monkeypoxvirus

Different kinds of media spiked with Monkeypoxvirus (MPXV) were subjected to heat inactivation at different temperatures, for various periods of time. Our results showed that MPXV was inactivated in less than 5 min at 70 {degrees}C and in less than 15 min at 60 {degrees}C, with no difference between virus from West African and Central African clades. Such indications could help laboratory workers to manipulate the virus in optimal biosafety conditions and improve their protocols.

microbiology↗

bioRxiv2020

Heat inactivation of the Severe Acute Respiratory Syndrome Coronavirus 2

Supernatants of cells infected with SARS-CoV-2, nasopharyngeal and sera samples containing SARS-CoV-2 were submitted to heat inactivation for various periods of time, ranging from 30 seconds to 60 minutes. Our results showed that SARS-CoV-2 could be inactivated in less than 30 minutes, 15 minutes and 3 minutes at 56{degrees}C, 65{degrees}C and 95{degrees}C respectively. These data could help laboratory workers to improve their protocols with handling of the virus in biosafety conditions.

microbiology↗