Biology subjects

Baron, D.

Publications and source records attributed to Baron, D..

3 recordsLinked to original sources

bioRxiv2026

Active teaching-learning methodology in a non-formal teaching environment as a tool to mitigate the "plant awareness disparity" in public school students.

Currently, a significant proportion of young people are discouraged by the teaching of botany and, consequently, show little interest in preserving local biodiversity. On the other hand, with the recurrent use of digital technologies (DTs), young people have shown significant interest in virtual devices. Our project's tested the hypothesis whether plants awareness disparity in public school students was overcome through active teaching and learning. Thus, the objective was to investigate the use of active methodology in a non-formal teaching environment (e.g., sensory garden, SG) in order to stimulate learning in plant organography based on its evaluation using digital games. Our proposal was investigated at the Lagoa do Sino campus (Buri/SP, Brazil) in partnership with the Public School System of the Municipality of Campina do Monte Alegre/SP, Brazil. Furthermore, students were divided into two teams, which applied qualitative methodologies, such as that one of these (Team A) had face-to-face contact with the SG, using the Problem-Based Learning (PBL) methodology, digital game and survey. Team B, on the other hand, will only have contact with the game. After collecting the data, we verified statistically significant differences between the teams using the Wilcoxon test and based on the latent traits evaluated. Given this, the literature points out that discouraging students influences their learning and, consequently, their education. In addition, our considerations are based on the latent trait knowledge of botany and, thus, we reject our null hypothesis and accept our alternative hypothesis. On the other hand, based on the latent trait of plant perception, we cannot affirm the acceptance or rejection of the hypotheses. It is worth noting that future studies should have a larger number of participants than this research in order to better evaluate what is intended.

scientific communication and education↗

bioRxiv2020

Noisy Pooled PCR for Virus Testing

Fast testing can help mitigate the coronavirus disease 2019 (COVID-19) pandemic. Despite their accuracy for single sample analysis, infectious diseases diagnostic tools, like RT-PCR, require substantial resources to test large populations. We develop a scalable approach for determining the viral status of pooled patient samples. Our approach converts group testing to a linear inverse problem, where false positives and negatives are interpreted as generated by a noisy communication channel, and a message passing algorithm estimates the illness status of patients. Numerical results reveal that our approach estimates patient illness using fewer pooled measurements than existing noisy group testing algorithms. Our approach can easily be extended to various applications, including where false negatives must be minimized. Finally, in a Utopian world we would have collaborated with RT-PCR experts; it is difficult to form such connections during a pandemic. We welcome new collaborators to reach out and help improve this work!

molecular biology↗

bioRxiv2018

FUS/TLS undergoes calcium-mediated nuclear egress during excitotoxic stress and is required for Gria2 mRNA processing

Excitotoxic levels of glutamate represent a physiological stress that is strongly linked to amyotrophic lateral sclerosis (ALS) and other neurological disorders. Emerging evidence indicates a role for neurodegenerative disease linked RNA-binding proteins (RBPs) in the cellular stress response. However, the relationships between excitotoxicity, RBP function and pathology have not been explored. Here, we found that excitotoxicity induced the translocation of select ALS-linked RBPs from the nucleus to the cytoplasm within neurons. RBPs affected by excitotoxicity include TAR DNA-binding protein 43 (TDP-43) and, most robustly, fused in sarcoma/translocated in liposarcoma (FUS/TLS). FUS translocation occurs through a calcium-dependent mechanism and coincides with striking alterations in nucleocytoplasmic transport. Further, glutamate-induced upregulation of Gria2 in neurons was dependent on FUS expression, consistent with a functional role for FUS under excitotoxic stress. These findings reveal a link between prominent factors in neurodegenerative disease, namely excitotoxicity, disease-associated RBPs and nucleocytoplasmic transport.

neuroscience↗