Neuroinflammation is an early pathological process in a mouse model of prodromal Parkinsons disease

Mercado, G.; Stetzik, L.; Schulz, E.; Meyerdirk, L.; Lindquist, A.; Cohen, L.; Escobar Galvis, M. L.; Steiner, J. A.; Moore, D. J.; Wesson, D. J.; Brundin, P.

doi:10.64898/2026.10.29.762888

bioRxiv · 10.64898/2026.10.29.762888

Neuroinflammation is an early pathological process in a mouse model of prodromal Parkinsons disease

Mercado, G.·Stetzik, L.·Schulz, E.·Meyerdirk, L.·Lindquist, A.·Cohen, L.·Escobar Galvis, M. L.·Steiner, J. A.·Moore, D. J.·Wesson, D. J.·Brundin, P.

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Abstract

Approximately 90% of Parkinson's disease (PD) patients exhibit olfactory deficits, which often precedes motor symptoms by years, and are a robust predictor for PD and subsequent cognitive decline. Pathological changes in the PD olfactory system, particularly synuclein (Syn) pathology in the olfactory bulb (OB) and severe involvement of the anterior olfactory nucleus (AON), are distinct from normal aging. However, the causal link between Syn aggregation and functional impairment, potentially involving cytotoxicity and neuroinflammation, remains elusive. To investigate these early pathological events, we utilized a prodromal PD model that we have previously characterized, involving the unilateral OB injection of Syn preformed fibrils (PFF) in wild-type mice. This triggers the transneuronal spread of pathogenic Syn, and olfactory dysfunction starting at 1-month post-injections. Here we report on neuropathological and inflammatory events 1-3 month following injection of PFFs into the OB. Following PFF injection, Syn pSer129 pathology progressively accumulated bilaterally in the AON and ipsilateral in the piriform cortex (PCX) over three months. Stereological quantification revealed no significant neuronal loss in the AON or PCX during this 1-3 month interval, indicating that olfactory impairment precedes overt neurodegeneration. Instead, we identified the upregulation of pro-inflammatory genes (Il-1{beta} ; and Il-6), significant microglial reactivity, and sustained astrogliosis within olfactory structures. These findings identify neuroinflammation as a key early mediator linking Syn pathology to olfactory dysfunction in the absence of neuronal loss. This suggests a critical window for therapeutic intervention and highlights neuroinflammatory markers as potential biomarkers for the prodromal phase of PD.

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Mercado, G., Stetzik, L., Schulz, E., Meyerdirk, L., Lindquist, A., Cohen, L., Escobar Galvis, M. L., Steiner, J. A., Moore, D. J., Wesson, D. J., Brundin, P.. 2026-10-30. Neuroinflammation is an early pathological process in a mouse model of prodromal Parkinsons disease. https://doi.org/10.64898/2026.10.29.762888

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