Breast Cancer-derived PDGFB Remodels the CNS Vascular Niche to Promote Brain, Spinal and Leptomeningeal Metastasis

Grabinski, N. S.; Reardon, J. J.; Shah, S.; Adorno, J. J.; Lee, P.; Packard, R. L.; Ramamoorthy, Y.; Thies, K. A.; Chakravarthy, P. A.; Agarwal, S. S.; Spehar, J. M.; Arsiwala, T. A.; Carpenter, J. W.; Mossing, A. A.; Schiebel, J. G.; Steck, S. A.; Didier, A. J.; Richardson, D. S.; Kollin, L.; Venere, M.; Godbout, J. P.; Lockman, P. R.; Beyer, S.; Corps, K. N.; Winter, J. O.; Song, J. W.; Sizemore, S. T.; Sizemore, G. M.

doi:10.64898/2026.10.15.759418

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bioRxiv · 10.64898/2026.10.15.759418

Breast Cancer-derived PDGFB Remodels the CNS Vascular Niche to Promote Brain, Spinal and Leptomeningeal Metastasis

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Abstract

Background: Central nervous system (CNS) metastases are a major driver of breast cancer (BC) mortality. Recently, our group demonstrated that platelet-derived growth factor-B (PDGFB) and its receptor, platelet-derived growth factor receptor-beta (PDGFR{beta}), promote BC metastasis to the brain; however, the cellular/molecular mechanisms driving this process remain unclear. Here, we investigated tumor-derived PDGFB effects on the CNS vascular niche in regulating metastatic spread. Methods: Triple-negative BC cells were engineered to overexpress PDGFB and used to evaluate endothelial sprouting, blood-brain barrier (BBB) function, vascular permeability and experimental CNS metastasis. A novel retro-orbital (RO) injection model was used to study leptomeningeal/spinal experimental metastasis. Results: PDGFB enhanced human brain microvascular endothelial cell (HBMEC) sprouting, induced cell migration in BBB spheroids, increased permeability in 3D models, and disrupted tight junction proteins in HBMECs. Notably, alterations in vascular permeability were observed in both PDGFR{beta}-positive and PDGFR{beta}-negative endothelial systems, suggesting PDGFB can remodel the CNS vasculature through multiple mechanisms. In vivo, PDGFB increased CNS metastatic burden and altered tumor vasculature in xenograft and allograft models of BC. Furthermore, PDGFB promoted robust colonization of both spinal and leptomeningeal compartments, identifying a previously underappreciated role for this signaling pathway in a clinically devastating form of CNS metastasis. Analysis of patient transcriptomic datasets revealed enrichment of angiogenesis-related pathways in PDGFB-high tumors from patients who developed CNS metastases. Conclusions: Collectively, these findings identify tumor-derived PDGFB as a key regulator of CNS vascular remodeling and metastatic tropism, while introducing a new experimental framework to study spinal metastases and leptomeningeal disease in BC.

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Grabinski, N. S., Reardon, J. J., Shah, S., Adorno, J. J., Lee, P., Packard, R. L., Ramamoorthy, Y., Thies, K. A., Chakravarthy, P. A., Agarwal, S. S., Spehar, J. M., Arsiwala, T. A., Carpenter, J. W., Mossing, A. A., Schiebel, J. G., Steck, S. A., Didier, A. J., Richardson, D. S., Kollin, L., Venere, M., Godbout, J. P., Lockman, P. R., Beyer, S., Corps, K. N., Winter, J. O., Song, J. W., Sizemore, S. T., Sizemore, G. M.. 2026-10-16. Breast Cancer-derived PDGFB Remodels the CNS Vascular Niche to Promote Brain, Spinal and Leptomeningeal Metastasis. https://doi.org/10.64898/2026.10.15.759418

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