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bioRxiv · 10.64898/2026.10.13.758534

A gαq signaling pathway in dopaminergic neurons suppresses memory

Martinez-Cervantes, J. C.·Shah, P.·Cervantes Sandoval, I.
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Abstract

Which experiences persist as memories is a fundamental question in neurobiology. The balance between memory persistence and suppression is thought to be optimal for an organism to adapt to the ever-changing environment. Memory suppression is a process by which the brain limits the strength and expression of memories. Memory suppressors are genes whose disruption can enhance memory at different stages: acquisition, consolidation, forgetting, or recall. Here, using aversive classical conditioning in Drosophila, we show that Gq acts as a memory suppressor in PPL1 dopaminergic neurons. Knockdown of gq in dopaminergic neurons enhances acquisition, strengthens anesthesia-resistant memory, and impairs reversal learning. In vivo functional imaging revealed that gq knockdown amplifies shock-evoked responses in a type of PPL1 neuron. Additionally, using ex vivo imaging and pharmacological experiments, we reveal that both Gq and the OAMB receptor alter octopamine-evoked ER calcium responses in PPL1 neurons. Our results uncover a new role of Gq signaling within dopaminergic neurons that suppresses memory.

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Martinez-Cervantes, J. C., Shah, P., Cervantes Sandoval, I.. 2026-10-14. A gαq signaling pathway in dopaminergic neurons suppresses memory. https://doi.org/10.64898/2026.10.13.758534

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