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bioRxiv · 10.64898/2026.10.02.753577

Associations between multivariate profiles, lifestyle behaviors and reaction time performance in active young adults

Valdivia-Tapia, G.·Huerta, P.·Vergara-Barra, P.·Fuentealba, J.·Castro, P. A.
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Abstract

Lifestyle behaviors influence cardiometabolic and cognitive health through multiple interconnected processes, involving metabolic, cardiovascular, behavioral and cognitive factors. This study aimed to assess associations between multivariate plasma and physiological profiles, and behavioral parameters, and their potential association with reaction time (RT) in active young adults. Data on physical activity levels were collected from wearable devices and GPAQ questionnaire, along with anthropometric and clinical assessments, cognitive tests, and blood analyses. Principal component analysis was performed separately for physiological and plasma parameters, retaining the first two principal components (PCs) from each analysis. Multiple linear regression models were used to evaluate the associations between plasma, sex, physical activity levels (METs), daily step count, and alcohol/marijuana use on physiological PC1/PC2 scores, as well as RT. Continuous independent variables were previously categorized, and combined variables were created to represent specific combinations between plasma/physiological PC1 categories and sex. Physiological PC1 (blood pressure) was associated with combined plasma PC1 (insulin/HOMA-IR)-sex variable, total METs and alcohol consumption (adjusted R2 = 0.30). Physiological PC2 (handgrip strength) was mainly explained by combined plasma PC1-sex variable (adjusted R2 = 0.54), with minimal contribution from behavioral factors. RT was associated with physiological PC1 variable and sex (adjusted R2 = 0.35), with slower responses observed among women with lower physiological PC1 scores or hypotension; these associations were supported by RT-physiological PC1 and RT-blood pressure correlations. These exploratory findings suggest sex-dependent associations between physiological, metabolic and lifestyle-related factors, supporting a holistic approach in future research, particularly among young adults.

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Valdivia-Tapia, G., Huerta, P., Vergara-Barra, P., Fuentealba, J., Castro, P. A.. 2026-10-09. Associations between multivariate profiles, lifestyle behaviors and reaction time performance in active young adults. https://doi.org/10.64898/2026.10.02.753577

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