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bioRxiv · 10.64898/2026.10.01.756049

SpatialOmicsLab: an integrated research environment for AI co-scientists in spatial transcriptomics

Jiang, Y.·Zhan, X.·Quan, P.·Wang, R.·Wu, F.·Mi, J.·Yao, J.·Yao, B.·Xiao, G.·Shi, W.·Xie, Y.
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Abstract

Spatial profiling is moving from method development into a central platform for biological discovery and clinical translation, but analyzing such complex data has become the bottleneck of scientific discovery. Translating spatial transcriptomic measurements into biological insight requires reproducible workflows that integrate diverse analytical methods. Although language-model agents can plan and execute multistep analyses, fragmented data and software ecosystems hinder their use as reliable research assistants. In this study, we introduce SpatialOmicsLab, an integrated research environment for spatial transcriptomics comprising 113 curated computational tools, 63 reference datasets and 4,614 task specifications. We also introduce ST-Coscientist, an AI co-scientist that uses this environment to assemble, execute and refine spatial transcriptomic analyses. Within SpatialOmicsLab, ST-Coscientist outperformed expert-led workflows and other scientific agents across six benchmark settings spanning spatial clustering, cell-type deconvolution and spatially variable gene detection. It constructed alignment-aware workflows for three-dimensional MERFISH data and addressed biological questions by selecting and analysing relevant reference datasets. Procedural memory increased the success rate of integrating and validating additional analytical tools from 48% to 95%. Together, SpatialOmicsLab and ST-Coscientist establish an extensible ecosystem for reproducible spatial transcriptomic research, enabling AI co-scientists to address evolving biological questions with an expanding analytical repertoire.

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Jiang, Y., Zhan, X., Quan, P., Wang, R., Wu, F., Mi, J., Yao, J., Yao, B., Xiao, G., Shi, W., Xie, Y.. 2026-10-08. SpatialOmicsLab: an integrated research environment for AI co-scientists in spatial transcriptomics. https://doi.org/10.64898/2026.10.01.756049

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