A subcellular spatial proteome of the non-photosynthetic euglenid Euglena longa

Abstract

Euglena longa is a heterotrophic model euglenid, of significant research interest as a unicellular eukaryote. To provide broad insight into the localisation of its proteome we used subcellular spatial proteomics (SSP), based on a label-free version of LOPIT-DC (Localisation of Organelle Proteins by Isotope Tagging after Differential Centrifugation), in which organelles are separated by differential centrifugation and each protein is assigned to a compartment from its abundance profile across the fractions. We detected 5,909 proteins, using protein abundance profiles across fractions to resolve 16 different subcellular compartments, such as the plastid, pellicle, and peroxisomes, and assigned high-confidence localisations to 2,955 of them. This study serves as a proteomic dataset to resolve protein compartmentalisation for this euglenid, enabling evolutionary, biological and functional insight and analysis. We are drafting manuscripts concerning the metabolic nature of the plastid, the evolutionary origins of the pellicle, and the functions of the peroxisome. Investigators interested in using this data prior to publication are encouraged to contact us to avoid overlap and to facilitate coordinated and collaborative use of this resource.