Limb Lengthening Induces Substantial Muscle Remodeling in Humans

Abstract

To correct lower limb length discrepancies, orthopedic surgeons often perform limb lengthening procedures. These procedures involve directly lengthening a primary bone (e.g., femur), which also stretches the muscles and tendons spanning the elongation site. Limb lengthening procedures improve lower-limb length symmetry, often at the expense of chronic joint-level decrements. For example, knee joint range of motion and strength does not always return to pre-surgery values following femur lengthening. These joint-level decrements governed by altered muscle force-length characteristics, but it is unestablished how human muscles remodel during surgical limb lengthening. Here, we characterized bilateral human vastus lateralis fascicle force-length profiles following femur lengthening. At 2.2 {+/-} 1.7 years (Avg {+/-} SE) post-initial surgery, the lengthened limb's vastus lateralis fascicles were longer, effectively stiffer, and weaker than the contralateral limb. The lengthened limb's vastus lateralis fascicles were 38 mm longer (Avg) than that of the contralateral limb, even though the lengthened limb's femur was only 31 mm longer (Avg). The onset of vastus lateralis fascicle passive force production began at 22% shorter (Avg) operating lengths in lengthened versus contralateral limb muscles. Additionally, vastus lateralis peak active force production was 34% less (Avg) in the lengthened versus contralateral limbs, and in-series quadriceps tendon stiffness was not stiffer in lengthened limbs. These data suggest that active muscle tissues lengthen more than passive muscle tissues following limb lengthening, effectively creating stiffer muscles. Years after limb lengthening, patient muscles exhibit numerous differences in their force-length properties versus that of natural growth, likely contributing to impaired joint-function.