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bioRxiv · 10.64898/2026.09.25.754324

Reduced Control Over Pavlovian-to-Instrumental Transfer in Human Addiction: A Systematic Investigation Across Alcohol Use Disorder and Smoking Status

Thill, L.·Chen, H.·Bäuchl, C.·Krasniqi, S.·Born, M.·Marxen, M.·Friedel, E.·Pilhatsch, M.·Heinz, A.·Smolka, M. N.
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Abstract

Pavlovian conditioned stimuli can compromise goal-directed behavior, particularly when they conflict with the required instrumental response. Previous research linked an increased impact of such Pavlovian-to-instrumental transfer (PIT) interference to risky drinking and alcohol dependence. However, it remained unclear whether the observed behavioral and neural alterations generalize to the substantially larger population of non-treatment-seeking individuals with predominantly mild-to-moderate alcohol use disorder (AUD) and to other substances like nicotine. We therefore studied 200 participants from the general population who were stratified by AUD and smoking status before completing a single-lever PIT task during functional imaging. PIT interference was measured as the difference in error rates between incongruent PIT trials, characterized by conflicts of Pavlovian and instrumental stimuli, and congruent trials. Associations with AUD and smoking status, as well as dimensional associations of behavioral PIT interference and neural responses to PIT conflicts with substance-related measures, were examined. As expected, participants with AUD showed greater PIT interference and reduced conflict-related activation in the lateral prefrontal, dorsomedial prefrontal, and anterior cingulate cortex than participants without AUD. The magnitude of these alterations also correlated with higher AUD severity and alcohol consumption, while being independent of smoking status and other smoking-related measures. These findings suggest that enhanced PIT interference generalizes to the broader AUD population but may not extend to other substances like nicotine. They further implicate a reduced recruitment of prefrontal control networks during PIT conflicts as a potential mechanism underlying enhanced PIT interference in AUD and its association with clinically relevant outcome measures.

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Thill, L., Chen, H., Bäuchl, C., Krasniqi, S., Born, M., Marxen, M., Friedel, E., Pilhatsch, M., Heinz, A., Smolka, M. N.. 2026-10-01. Reduced Control Over Pavlovian-to-Instrumental Transfer in Human Addiction: A Systematic Investigation Across Alcohol Use Disorder and Smoking Status. https://doi.org/10.64898/2026.09.25.754324

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