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bioRxiv · 10.64898/2026.09.20.752925

CytosMPS: an automated multimodal pooled screening platform for protein, morphology and transcriptomics

Lopez, T.·Rabalais, J.·Song, B.·Boddicker, A.·Schwartz, A.·Honigfort, D.·Zhao, J.·Kellinger, M.·Kruglyak, S.
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Abstract

Cytos Multimodal Pooled Screening (CytosMPS) measures CRISPR guide identity, morphology, native 3' transcriptome, and protein abundance and localization in the same fixed cells. Following IL-1{beta} stimulation, cells with higher nuclear NF-{kappa}B show lower phospho-p38/HSP27 and higher NFKBIA/DUSP1 in the same cell, a measurement neither imaging nor transcriptomics alone provides. TGFBR2 and CTNNB1 perturbations produce distinct multimodal phenotypes. Guide representation and perturbation transcriptional responses agree with an orthogonal single-cell transcriptomic platform.

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Lopez, T., Rabalais, J., Song, B., Boddicker, A., Schwartz, A., Honigfort, D., Zhao, J., Kellinger, M., Kruglyak, S.. 2026-09-24. CytosMPS: an automated multimodal pooled screening platform for protein, morphology and transcriptomics. https://doi.org/10.64898/2026.09.20.752925

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