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bioRxiv · 10.64898/2026.09.17.752430

GLOBAL ANALYSIS OF PROTRUSION-TRANCSRIPTOMES IDENTIFIES DISTINCT CATEGORIES OF LOCALIZED RNAS IN NON-NEURONAL CELLS

Hojo, B.·Kumari, S.·Milla, D.·Vang, V.·Norris, M. L.
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Abstract

RNA localization to protrusions in non-neuronal cells is an emerging molecular process, distinct from the canonical mechanism characterized in neurons. Here, we describe a fractionation workflow optimized for non-neuronal cells that increases sensitivity and reproducibility of transcriptome-wide quantification of protrusion-localized RNAs. Using the optimized method, we identify six categories of protrusion-localized RNAs in non-neuronal cells from mice, including long non-coding RNAs and pseudogenes, and observe spatially distinct subsets of mitochondrially-localized RNAs. Taken together, our results reveal previously unappreciated spatial regulation of diverse transcripts and point toward broadly acting unifying principles that extend across cell types and species.

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Hojo, B., Kumari, S., Milla, D., Vang, V., Norris, M. L.. 2026-09-18. GLOBAL ANALYSIS OF PROTRUSION-TRANCSRIPTOMES IDENTIFIES DISTINCT CATEGORIES OF LOCALIZED RNAS IN NON-NEURONAL CELLS. https://doi.org/10.64898/2026.09.17.752430

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