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bioRxiv · 10.64898/2026.09.11.749817

Beyond Conveyance: Assessing the Contribution of Process-Critical Bacteria from Urban Sewer Systems to Wastewater Treatment Plants

Maia Valenca, R.·Riisgard-Jensen, M.·Haaning Nielsen, A.·Halkjaer Nielsen, P.·Peces, M.
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Abstract

The sewer microbiome is known to be the main source of process-critical bacteria involved in nitrogen and phosphorus removal in wastewater treatment plants (WWTPs) and is thus crucial for the assembly of activated sludge (AS) microbial communities. However, the extent of these bacterial loads arriving at WWTPs currently lacks scientific attention. We provide a quantitative description of the biomass loads from sewer systems to WWTPs by coupling microbial community data by 16S rRNA amplicon sequencing from the Aalborg municipality sewer catchment with mass balances and biomass production across gravity and pressure sewer systems. Accounting for microbial transformations in sewer wastewater, biofilm and sediments under steady state dry weather flow conditions, gravity sewers were found to produce higher biomass loads than pressure systems, with loads of 1.57 and 0.27 kgCOD m-3 d-1 respectively. A key feature of our sewer model is its ability to estimate biomass loads from specific taxa, such as polyphosphate accumulating organisms (PAOs). Using Aalborg West WWTP as a case study, model estimations correlated well with figures calculated based on influent wastewater data monitoring. The model-estimated loads for the PAOs Azonexus and Ca. Accumulibacter were 2.10 kg day-1 and 1.80 kg day-1, compared to the influent wastewater monitoring-based loads of 1.77 kg day-1 and 1.20 kg day-1, respectively. While providing a framework for further research on sewer microbial dynamics, this study offers a tool for gaining insights into microbial immigration, generating valuable information for improving microbial communities management in WWTPs, optimizing nutrient removal, and enhancing WWTP design.

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Maia Valenca, R., Riisgard-Jensen, M., Haaning Nielsen, A., Halkjaer Nielsen, P., Peces, M.. 2026-09-11. Beyond Conveyance: Assessing the Contribution of Process-Critical Bacteria from Urban Sewer Systems to Wastewater Treatment Plants. https://doi.org/10.64898/2026.09.11.749817

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