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bioRxiv · 10.64898/2026.09.10.750597

roostR: An R package to examine diel activity patterns from Motus radio telemetry data

Williams, K. A.·Morgan, J. E.·Lyons, S. M.
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Abstract

1. Monitoring the diel activity patterns of free-living animals is a methodological challenge. Signal strength fluctuations from Very High Frequency (VHF) radio transmitters deployed within the Motus Wildlife Tracking System can be used as a proxy for activity. However, analytical tools to extract behavioral metrics that quantify activity patterns from these data are needed. 2. We developed roostR, an open-source R package that converts Motus detection data into quantitative behavioral metrics, including roost initiation and departure, roost duration, observation time, and restlessness. The package uses a sequential pipeline built around signal volatility and rolling medians to detect transitions between active and inactive states. Default parameter values were tuned using data from 55 dark-eyed juncos (Junco hyemalis) overwintering in southeastern Ohio. 3. We provide an example from a dark-eyed junco over a 58-day period. roostR estimated roost onset on 48 nights and departure on 56 mornings, with higher rolling median signal differences during the day than at night, consistent with a diurnal animal, and variable restlessness periods each night. We also used the package to estimate roost behavior of an American tree sparrow (Spizelloides arborea) over 43-nights. 4. roostR enables researchers to extract individual activity data from Motus datasets. Because all thresholds are user-adjustable, the pipeline is adaptable across species, tag specifications, and ecological contexts, enabling researchers to test hypotheses about how environmental factors influence diel activity patterns.

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Williams, K. A., Morgan, J. E., Lyons, S. M.. 2026-09-15. roostR: An R package to examine diel activity patterns from Motus radio telemetry data. https://doi.org/10.64898/2026.09.10.750597

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