bioRxiv Science⌕ Search

bioRxiv · 10.64898/2026.09.08.747031

Calibrating Classifiers Across Covariates: Hierarchical Vocal Density Estimation for Acoustic Monitoring at Scale

Abstract

Passive acoustic sensors and machine learning classifiers offer a scalable approach to monitoring wildlife populations. These classifiers typically detect whether a species is present in a short time-window of audio; ecologists, however, want ecologically meaningful measures such as indices of site-level abundance. Vocal density, the proportion of time-windows containing a vocalization, is a useful abundance proxy. Recovering vocal density from classifiers requires calibrating their outputs against expert-labeled data. Because classifier performance varies between sites, a single global calibration yields overconfident site-level estimates, yet calibrating every site independently requires prohibitive labeling effort. Estimating site-level vocal density at the scale of modern sensor networks demands a label-efficient alternative. We present a Bayesian hierarchical extension of Platt scaling, a commonly used calibration method, that calibrates classifiers at the site level while borrowing strength across sites. Where site-level covariates are available, a Gaussian-process prior lets the model learn how calibration varies across covariate space. Averaging calibrated per-clip probabilities across a site's recordings yields a posterior distribution over vocal density, and we show how to carry that uncertainty into downstream regressions of vocal density on environmental covariates. Across simulations and two fully annotated field datasets from Hawai'i and the Pacific Northwest, our model attained desired credible-interval coverage of vocal density estimates with narrower intervals than independent site-level calibration at equal labeling effort, and lower mean squared error. Global calibration, by contrast, failed to attain coverage except when simulated sites were genuinely homogeneous. Unlike site-level calibration, our model enables calibration at sites with zero labeled data, and incorporating covariates further improved precision when site heterogeneity was covariate-driven. Applied to a 283-site dataset in Pennsylvania with only two labeled clips per site, we recovered known habitat associations for the declining Wood Thrush (Hylocichla mustelina). Our method provides a principled, label-efficient route from bioacoustic classifier scores to site-level abundance indices with well-quantified uncertainty, making rigorous ecological inference feasible at the scale at which acoustic sensor networks are now deployed.

Explore related subjects

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Freeland-Haynes, L., Weldy, M. J., Navine, A. K., Hart, P. J., Kitzes, J., Denton, T.. 2026-09-12. Calibrating Classifiers Across Covariates: Hierarchical Vocal Density Estimation for Acoustic Monitoring at Scale. https://doi.org/10.64898/2026.09.08.747031

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

A leaf-carbon economy model to explain the adaptive value of low vs. high leaf mass per area as a function of habitat productivity

Plant traits interact with environmental conditions to determine plant fitness. In particular, plant species with low leaf mass per area (LMA) are more common in high fertility soils and forest gaps, while plant species with high LMA are more common in low fertility soils and in shaded forest understories. It is not yet known why the adaptive value of LMA changes as a function of environmental productivity. We present a model for plant growth as a function of LMA and productivity. Our model relies on an empirically derived relationship between LMA and leaf lifespan. We are able to explain why species with lower LMA are successful in high productivity environments including environments with high availability of either light or soil nutrients, by showing that the LMA which maximizes growth rate decreases with increasing rate of carbon assimilation. We show that low LMA species are better at taking advantage of the 'high interest' environment of high productivity habitats by reinvesting assimilated carbon in new leaves and earning compound interest, while high LMA species grow more efficiently in the 'low interest' environment of low productivity habitats. This tradeoff sheds light on the adaptive value of LMA as it relates to environmental productivity.

ecology↗

Species differentiate water uptake depths within a narrow vertical zone in temperate grasslands

Whether and under which conditions coexisting species in temperate grasslands differentiate their water uptake within the shallow soil layer they share is central to understanding hydrological niche differentiation in these ecosystems. In this field study, we quantified water uptake depth of 28 coexisting herbaceous species across 74 temperate grassland plots in three regions of Germany using oxygen stable isotopes and soil water content measured at multiple depths. Species consistently differed in water uptake depth across all regions, consistent with hydrological niche differentiation. This differentiation was largely confined to the upper 30 cm of soil and became stronger as overall soil moisture declined, with plants accessing water at greater depths in drier plots. In contrast, the vertical distribution of soil moisture was unrelated to either uptake depth or species differentiation. Despite pronounced species level variation, broad herbaceous functional classifications, including grasses versus forbs and fibrous versus taprooted species, showed substantial overlap in water uptake depth. Together, our results show that hydrological niche differentiation in temperate grasslands is expressed through fine scale differences among species within a shared shallow soil domain, becomes more pronounced under drier conditions, and is poorly captured by broad herbaceous functional classifications.

ecology↗

Fungal-bacterial interactions enhance bacterial but constrain fungal resistance and recovery to extreme heat across soil moisture levels

Soil fungi and bacteria are key drivers of nutrient cycling and plant productivity in terrestrial ecosystems, yet their responses to climate extremes are rarely studied as interacting partners rather than in isolation. Moreover, it remains largely unknown how fungal-bacterial interactions regulate fungal and bacterial stability under climate extremes, particularly resistance (immediate responses to climate extremes) and recovery (responses after climate extremes cease). Here, we conducted a soil microcosm experiment with a bacterial species (Pseudomonas putida KT2440) paired with one of four Trichoderma species (T. hamatum, T. atroviride, T. koningiopsis, and T. harzianum). These fungal-bacterial pairs were then exposed to a 5-day heat event (40 {degrees}C/32 {degrees}C, day/night) followed by a 14-day recovery period (25 {degrees}C/17 {degrees}C, day/night), at two soil moisture levels (30% and 5% gravimetric water content). Our results show that fungal-bacterial interactions asymmetrically affect fungal and bacterial responses to extreme heat. Trichoderma species facilitated the resistance and recovery of P. putida density (CFUs) to extreme heat, whereas P. putida constrained the resistance and recovery of fungal biomass (ergosterol) to extreme heat, regardless of soil moisture conditions. Moreover, faster-growing Trichoderma species were associated with greater facilitation of bacterial resistance and recovery. Bacterial density also covaried positively with fungal biomass during heat stress under both soil moisture conditions, suggesting that net fungal facilitation enhances bacterial persistence under extreme heat. Together, our findings reveal that fungal-bacterial interactions can stabilize one microbial partner while increasing the vulnerability of the other, highlighting the contrasting consequences of biotic interactions under climate extremes.

ecology↗