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bioRxiv · 10.64898/2026.09.04.749539

Stepwise bladder carcinogenesis reveals transcriptional reprogramming involving MMP1 and COL7A1

Nagashima, Y.·Elbadawy, M.·Naito, Y.·Abugomaa, A.·Tsunedomi, R.·Kaneda, M.·Usui, T.·Sasaki, K.
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Abstract

Bladder cancer develops through multistep molecular alterations, yet the events underlying tumor initiation and progression remain poorly understood. Here, we reconstructed stepwise bladder carcinogenesis using canine bladder organoids combined with chemical carcinogenesis and serial xenotransplantation. Carcinogen-exposed normal organoids generated benign tumors that subsequently progressed to invasive carcinomas. Despite a low tumor mutational burden and limited acquisition of cancer-associated mutations, malignant progression was accompanied by extensive transcriptional reprogramming, including enrichment of epithelial-to-mesenchymal transition programs. COL7A1 and MMP1 were progressively upregulated during progression, and knockdown of either gene suppressed organoid proliferation and invasion. These functional dependencies were reproduced in independently established spontaneous canine bladder cancer organoids, where silencing of either gene also reduced xenograft growth and mitosis-related gene programs. MMP1 knockdown decreased COL7A1 expression, suggesting a potential regulatory relationship. MMP1 was also elevated in basal/squamous human bladder cancers, and its depletion suppressed human bladder cancer cell proliferation. These findings identify transcriptional reprogramming and MMP1/COL7A1 dependencies as prominent features of malignant progression.

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Nagashima, Y., Elbadawy, M., Naito, Y., Abugomaa, A., Tsunedomi, R., Kaneda, M., Usui, T., Sasaki, K.. 2026-09-09. Stepwise bladder carcinogenesis reveals transcriptional reprogramming involving MMP1 and COL7A1. https://doi.org/10.64898/2026.09.04.749539

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