Cell-state-dependent responses to PLK1 inhibition reveal a non-canonical microtubule-endolysosomal vulnerability in quiescent leukemia stem cells

Liu, Q.; Gojsevic, M.; Varesi, A.; Subedi, A.; Xu, C.; Yeung, F. A.; Dinel, B.; Mbong, N.; Jin, L.; Mitchell, A.; Lim, C.; Boutzen, H.; Arruda, A.; Minden, M. D.; Lechman, E. R.; Raught, B.; Chan, S. N.; Bader, G. D.; Kaufmann, K. B.; Wang, J. C.

doi:10.64898/2026.08.25.746864

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bioRxiv · 10.64898/2026.08.25.746864

Cell-state-dependent responses to PLK1 inhibition reveal a non-canonical microtubule-endolysosomal vulnerability in quiescent leukemia stem cells

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Abstract

Relapse in cancer is frequently driven by therapy-resistant quiescent cancer stem cells. Conventional chemotherapy has been designed to target proliferating tumor cells and is generally presumed to be ineffective against non-cycling cancer stem cells. Using acute myeloid leukemia (AML) as a model, we challenge this prevailing view by showing that inhibition of the mitotic master regulator Polo-like kinase 1 (PLK1), a kinase extensively pursued for antiproliferative cancer therapy, unexpectedly eradicates quiescent leukemia stem cells (LSC) through a mechanism distinct from its canonical mitotic function. In proliferating AML cells, PLK1 inhibition (PLK1i) induced G2/M arrest and mitotic catastrophe. In contrast, quiescent LSC underwent apoptosis independent of mitotic arrest, revealing a cell-state-dependent mode of drug action. Mechanistically, PLK1i initiated a multi-step process through disruption of a previously unrecognized, stem cell-specific interaction between PLK1 and MAP1A, resulting in perturbed vesicle trafficking and endolysosomal homeostasis characterized by altered receptor internalization, vesicle accumulation and lysosomal dysfunction, ultimately culminating in apoptotic cell death. Combinatorial pharmacologic perturbation studies established microtubule regulation as a critical determinant of quiescent LSC survival, while ex vivo and in vivo assays demonstrated depletion of functionally-defined LSC following PLK1i. These findings identify a previously unrecognized role for PLK1 in intracellular trafficking and establish MAP1A-dependent control of vesicle homeostasis as a mechanistic determinant of cancer stem cell survival. More broadly, this study demonstrates that classical antimitotic compounds, including microtubule-targeting agents and PLK1 inhibitors, can eradicate both cycling leukemic blasts and quiescent LSC through distinct, cell state-dependent mechanisms, challenging proliferation-centric models of chemotherapy action.

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Liu, Q., Gojsevic, M., Varesi, A., Subedi, A., Xu, C., Yeung, F. A., Dinel, B., Mbong, N., Jin, L., Mitchell, A., Lim, C., Boutzen, H., Arruda, A., Minden, M. D., Lechman, E. R., Raught, B., Chan, S. N., Bader, G. D., Kaufmann, K. B., Wang, J. C.. 2026-08-26. Cell-state-dependent responses to PLK1 inhibition reveal a non-canonical microtubule-endolysosomal vulnerability in quiescent leukemia stem cells. https://doi.org/10.64898/2026.08.25.746864

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