The circadian system is affected by Alzheimers disease independently from amyloid beta deposits

Calligaro, H.; Khov, B.; Noel, K.; Glina, A.; van Rosmalen, L.; Ramasamy, R.; Li, Y.; Lam, M. T. Y.; Le, H.; Kim, K.-Y.; Ju, W.-K.; Ellisman, M.; Panda, S.

doi:10.64898/2026.08.25.744599

bioRxiv · 10.64898/2026.08.25.744599

The circadian system is affected by Alzheimers disease independently from amyloid beta deposits

Calligaro, H.·Khov, B.·Noel, K.·Glina, A.·van Rosmalen, L.·Ramasamy, R.·Li, Y.·Lam, M. T. Y.·Le, H.·Kim, K.-Y.·Ju, W.-K.·Ellisman, M.·Panda, S.

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Abstract

Circadian disruption, notably sleep disturbances, serves as an early indicator of Alzheimers disease (AD), preceding cognitive symptoms like memory loss. The suprachiasmatic nucleus (SCN) governs biological rhythms and receives direct retinal input via melanopsin-expressing retinal ganglion cells (mRGCs) to synchronize with environmental light cycles. The anatomical and functional basis for circadian disruption in AD remains unclear. Here, we explored the multi-level relationships between gene expression, the SCN connectome, and regulations of sleep and circadian rhythms in the APP/PS1 mouse model. The sleep architecture of APP/PS1 mice displayed significantly reduced rapid eye movement sleep (REM), associated with a reduced daily core body temperature amplitude and locomotor hyperactivity. Lastly, APP/PS1 mice showed an impaired response to acute light pulse stimulation and present hyperactivity of mRGCs at a young age and hypoactivity of these cells at older ages. These physiological functions are known to be, at least in part, regulated by the SCN, the main target of mRGCs. We noted several modifications in SCN connectomics using serial blockface electron microscopy (SBEM), including a reduction of the dendro-dendritic chemical synapse (DDCS) network that receives a large part of the retinal input and is thought to be crucial for synchronicity between SCN neurons. In addition, we observed multiple signs of dystrophy, including modifications of the shape of dendrites and cell soma, accumulation of aggregated lysosomes, and swelling of axons. At the same time, we investigated the changes in gene expression using spatial transcriptomics. The SCN presents changes in the expression of genes associated with synapse formation, cell adhesion, and neurite growth. These results suggest that, despite the absence of amyloid plaques in the ventral hypothalamus, the SCN of APP/PS1 mice still undergo profound gene expression changes, impacting connectomics and physiological functions. Graphical abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=157 SRC="FIGDIR/small/744599v1_ufig1.gif" ALT="Figure 1"> View larger version (39K): org.highwire.dtl.DTLVardef@ceedb0org.highwire.dtl.DTLVardef@156cfaaorg.highwire.dtl.DTLVardef@5bc262org.highwire.dtl.DTLVardef@36df4d_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Calligaro, H., Khov, B., Noel, K., Glina, A., van Rosmalen, L., Ramasamy, R., Li, Y., Lam, M. T. Y., Le, H., Kim, K.-Y., Ju, W.-K., Ellisman, M., Panda, S.. 2026-09-01. The circadian system is affected by Alzheimers disease independently from amyloid beta deposits. https://doi.org/10.64898/2026.08.25.744599

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