The RND family efflux pump FemT contributes to lipid homeostasis in Staphylococcus aureus

Thukral, A.; Bonn Dunbar, C. M.; Halucha, J.; Schneider, J. E.; Pereira, T. R.; McCormick, J. K.; Heinrichs, D. E.; McGavin, M. J.

doi:10.64898/2026.08.10.744025

bioRxiv · 10.64898/2026.08.10.744025

The RND family efflux pump FemT contributes to lipid homeostasis in Staphylococcus aureus

Thukral, A.·Bonn Dunbar, C. M.·Halucha, J.·Schneider, J. E.·Pereira, T. R.·McCormick, J. K.·Heinrichs, D. E.·McGavin, M. J.

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Abstract

The RND efflux pump FemT encoded by SAUSA300_2213 of Staphylococcus aureus USA300 is co-transcribed with femX which has an essential role in synthesizing the Lipid II precursor of peptidoglycan. Anticipating that this arrangement reflects a critical accessory role for femT, we constructed USA300{Delta}femT to assess its function. Although growth of USA300{Delta}femT in tryptic soy broth (TSB) was not impaired, transcriptomic data revealed a mild cellular stress response, accompanied by reduced expression of ohyA and crt genes involved in fatty acid metabolism and carotenoid lipid synthesis respectively. Accordingly, USA300{Delta}femT exhibited impaired growth on exposure to saturated and unsaturated fatty acids, and exposure to subinhibitory 50 {micro}M palmitic acid promoted accumulation of reactive oxygen species, reduced respiratory activity, and altered membrane function and morphology. The transcriptome of cells grown under this condition revealed strongly attenuated expression of ohyA and crt, and several genes required for oxidative and anaerobic respiration, concomitant with strongly enhanced expression of several stress response pathways. Cellular metabolites were also profoundly altered. Finally, lipidomic analysis of USA300{Delta}femT exposed to oleic acid revealed increased incorporation of oleic acid into phosphatidylglycerol, accompanied by a significant reduction in undecaprenol C55 lipid carrier, and respiratory quinones MK-7 and MK-8. Our data are consistent with a role for FemT in maintaining cellular lipid homeostasis by promoting efflux of isoprenoid and carotenoid lipids that are prone to oxidative damage, including C55 and menaquinones that undergo cyclic reactions in peptidoglycan synthesis and electron transport.

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Thukral, A., Bonn Dunbar, C. M., Halucha, J., Schneider, J. E., Pereira, T. R., McCormick, J. K., Heinrichs, D. E., McGavin, M. J.. 2026-08-11. The RND family efflux pump FemT contributes to lipid homeostasis in Staphylococcus aureus. https://doi.org/10.64898/2026.08.10.744025

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