Ferritinophagy Contributes to Iron Accumulation and Ferroptosis in FuchsEndothelial Corneal Dystrophy

Shepard, Z.; Skeie, J. M.; Shevalye, H.; Eggleston, T.; Li, L.; Field, M.; Schmidt, G.; Phruttiwanichakun, P.; Sales, C.; Salem, A. K.; Greiner, M.

doi:10.64898/2026.08.08.743691

bioRxiv · 10.64898/2026.08.08.743691

Ferritinophagy Contributes to Iron Accumulation and Ferroptosis in FuchsEndothelial Corneal Dystrophy

Shepard, Z.·Skeie, J. M.·Shevalye, H.·Eggleston, T.·Li, L.·Field, M.·Schmidt, G.·Phruttiwanichakun, P.·Sales, C.·Salem, A. K.·Greiner, M.

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Abstract

PurposeFuchs endothelial corneal dystrophy (FECD) is a progressive disease, causing premature death of corneal endothelial cells (CECs). Iron-dependent lipid peroxidation and ferroptosis mediate cell death in FECD. We aimed to determine whether FECD progression is mediated by derangements in ferritinophagy - a form of autophagy that degrades ferritin to release labile ferrous iron - and whether ultraviolet A (UVA) exposure drives FECD progression by activating ferritinophagy. MethodsEndothelium-Descemet membrane (EDM) tissues were collected from patients with end-stage FECD undergoing endothelial keratoplasty and from healthy age-matched donor corneas. Separately, immortalized FECD and healthy control CEC lines were cultured. Cellular levels of NCOA4 production and LC3 activation, both markers of ferritinophagy, were quantified using western blotting and PCR. UVA-exposed immortalized cells were plated on coverslips, stained for immunohistochemistry (IHC), and analyzed using confocal microscopy. Corneal endothelial peels were stained and analyzed using laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). ResultsSurgically explanted FECD CECs showed significantly increased levels of NCOA4 compared to healthy controls. LC3 activation was increased in FECD immortalized CECs; UV exposure further increased LC3 activation. Additionally, UVA exposure showed trends of increased expression of NCOA4 in immortalized FECD and healthy CECs. On IHC of FECD surgical explant tissue, ferritin was decreased markedly, NCOA4 localized in a dramatic punctate pattern, and both ferritin and LC3 localized within cell nuclei. Spectrometry images showed higher iron levels correlating with areas of higher FECD disease burden. ConclusionsOur results demonstrate ferritinophagy in FECD indicated by the increase of NCOA4 and LC3 ferritinophagy markers in FECD patient and cell culture models. Our finding that UVA activates ferritinophagy implicates this mechanism in UVA-mediated FECD progression. Altogether, aberrant iron dysregulation associated with FECD and ferroptosis may be mediated by ferritinophagy, providing a biomarker to assess disease severity as well as a potential target for future medical therapeutics.

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Shepard, Z., Skeie, J. M., Shevalye, H., Eggleston, T., Li, L., Field, M., Schmidt, G., Phruttiwanichakun, P., Sales, C., Salem, A. K., Greiner, M.. 2026-08-10. Ferritinophagy Contributes to Iron Accumulation and Ferroptosis in FuchsEndothelial Corneal Dystrophy. https://doi.org/10.64898/2026.08.08.743691

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