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bioRxiv · 10.64898/2026.08.07.743462

Prime-Editing in Marchantia paleacea: Expanding the Genome-Editing Toolbox in Bryophytes

Danilo, B.·Quillien, A.·Rojas-Latorre, C.·Nibani, Z.·Mestre, C.·Delaux, P.-M.·Lauressergues, D.·Neveu, J.
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Abstract

Since the development of CRISPR-based genome editing tools, a number of novel technologies have emerged. This includes Prime-Editing that acts as a search and replace genome editing tool. Prime-Editing has been deployed across multiple clades, including in a few flowering plants. Here, we report on the development of an efficient Prime Editor (PE) for the model bryophyte Marchantia. Initial tests were conducted on Acetolactate Synthase as a target and revealed an average efficiency above 40%. The system has been developed in the GoldenGate cloning system, facilitating construct design. The development of PE in Marchantia expands the Genome-Editing tools available for this emerging model in plant biology.

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Danilo, B., Quillien, A., Rojas-Latorre, C., Nibani, Z., Mestre, C., Delaux, P.-M., Lauressergues, D., Neveu, J.. 2026-08-11. Prime-Editing in Marchantia paleacea: Expanding the Genome-Editing Toolbox in Bryophytes. https://doi.org/10.64898/2026.08.07.743462

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