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bioRxiv · 10.64898/2026.08.06.743304

Zebrafish prph2a/b and rom1a/b serve distinct functions during cone and rod outer-segment assembly.

Patel, M.·Famulski, J.
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Abstract

Inherited retinal disorders are significant contributors of blindness worldwide. Mutations in Peripherin-2 (PRPH2), a highly conserved vertebrate tetraspanin membrane protein responsible for formation and maintenance of OS morphology, have been shown to cause diverse types of inherited photoreceptor cell (PRC) disorders including but not limited to Leber congenital amaurosis, cone-rod dystrophy, and retinitis pigmentosa. In this study we used a cone-rich diurnal zebrafish model to characterize the loss of PRPH2 function. Of the four PRPH2 zebrafish orthologs only prph2a and prph2b were found to be expressed in PRCs. CRISPR-mediated single mutants of prph2a and prph2b did not yield striking rod or cone phenotypes. Double prph2a/2b mutants exhibited early loss of all cone cells, preceded by cone outer segment disorganization in the form of whorls akin to the phenotypes observed in PRPH2+/- mice. Surprisingly rod photoreceptor cells were not affected and in fact exhibited a striking lengthening of rod OSs with normal disc formation. Overgrowth of rod OSs proceeded up to 1 year, but no degeneration was observed. To determine how rod OS can persist without prhp2a/b we targeted rom1a and rom1b using CRISPR. Injection of rom1a/b crRNA resulted in complete loss of both rod and cone OSs in the prph2a/b double mutants. Surprisingly, inhibition of rom1a/b alone resulted in the loss of rod but not cone OSs. These findings suggest that unlike in mammals, zebrafish rom1a/b is essential for rod OS formation while prph2a/b is essential for cone OSs.

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Patel, M., Famulski, J.. 2026-08-07. Zebrafish prph2a/b and rom1a/b serve distinct functions during cone and rod outer-segment assembly.. https://doi.org/10.64898/2026.08.06.743304

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