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bioRxiv · 10.64898/2026.08.01.742206

Mechanism of branched actin assembly at microtubule tips downstream of Adenomatous Polyposis Coli (APC) protein

Fang, X.·Efimova, N.·Svitkina, T.
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Abstract

During cell migration, branched actin filaments nucleated by the Arp2/3 complex induce leading edge protrusions, whereas directionality of cell migration is controlled by microtubules. We showed previously that Adenomatous Polyposis Coli (APC) initiates branched actin assembly at microtubule tips, which can explain how microtubules control directional protrusion. Here, we investigate a link from APC to Arp2/3 complex activity. We show that protrusion-generating activity of APC resides in its N-terminal region containing the Armadillo Repeat Domain (ARD). Furthermore, Asef1/ARHGEF4, a Cdc42 GEF known to be activated by the ARD of APC, as well as Cdc42 itself and its effector N-WASP, an Arp2/3 complex activator, are all required for the assembly of branched actin filaments in neuronal growth cones and neurite outgrowth. As downregulation of Asef1, Cdc42 or N-WASP produces phenotypes similar to APC knockdown, we propose that microtubules regulate directional cell migration and neuron navigation by inducing local membrane protrusion through the APC - Asef1 - Cdc42 - N-WASP - Arp2/3 pathway. These findings bridge a significant gap in our knowledge of cell migration.

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Fang, X., Efimova, N., Svitkina, T.. 2026-08-03. Mechanism of branched actin assembly at microtubule tips downstream of Adenomatous Polyposis Coli (APC) protein. https://doi.org/10.64898/2026.08.01.742206

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