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bioRxiv · 10.64898/2026.07.25.740702

Targeted measurement of blood flow in the Anterior Cerebral Artery using Diffuse Correlation Spectroscopy

Das, S.·Sharma, K.·Sarkar, S.·Gonsalves, K.·Srinivasan, U. S.·VARMA, H.
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Abstract

SignificanceDiffuse Correlation Spectroscopy (DCS) is an established technique for non-invasive monitoring of Cerebral Blood Flow (CBF), but existing applications primarily measure CBF changes averaged over cortical tissue volumes. A method capable of targeting blood flow within a particular intracranial artery would expand the utility of DCS for vessel-specific cerebral perfusion monitoring and continuous bedside assessment. AimWe aim to investigate the feasibility of targeted, non-invasive monitoring of blood flow in the Anterior Cerebral Artery (ACA) using DCS through optimization of probe geometry and placement. ApproachA custom-built DCS system operating at 785 nm was used to probe ACA from the glabellar region. Source-detector (SD) separation, probe orientation, and probe location were systematically optimized using lower-limb motor tasks and a mental arithmetic task. The optimized configuration was evaluated using an ACA-mimicking multilayer phantom and validated in forty healthy volunteers during lower-limb activation tasks and postural changes. ResultsAn SD separation of 17 mm, vertical probe orientation, and glabellar placement provided the highest sensitivity to ACA-related blood flow changes. Phantom experiments demonstrated sensitivity to flow changes in a vessel located 45 mm beneath the scalp and showed that the frontal sinus, cerebrospinal fluid, and the absence of cortical tissue beneath the glabella along the longitudinal fissure together provide the best optical window for probing deep ACA flow. Using the optimized probe configuration, significant increases in relative CBF were observed during standing leg marching (66.4 {+/-} 38.6%) and supine leg crunches (39.4 {+/-} 32.2%) (p < 0.01), with consistent responses during supine-to-stand postural transitions. ConclusionThe proposed DCS approach enables targeted measurement of blood flow within the ACA territory. This technique provides a framework for continuous, non-invasive monitoring of ACA perfusion and has potential applications in cerebrovascular monitoring and stroke care.

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Das, S., Sharma, K., Sarkar, S., Gonsalves, K., Srinivasan, U. S., VARMA, H.. 2026-07-27. Targeted measurement of blood flow in the Anterior Cerebral Artery using Diffuse Correlation Spectroscopy. https://doi.org/10.64898/2026.07.25.740702

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