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bioRxiv · 10.64898/2026.07.25.740310

Effects of different shading levels on growth, physiology and leaf surface micromorphology of Sorbus sibirica "Dong Hong" seedlings

Huang, R.·Gong, W.·Li, X.·Ji, S.·Cui, T.·Zhang, L.
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Abstract

BackgroundSorbus sibirica Dong Hong is a promising ornamental cultivar, but its optimal light conditions remain unclear. AimsThis study evaluated the effects of shading on seedling growth, physiology, root morphology, and leaf surface micromorphology. MethodsOne-year-old seedlings were grown under full sunlight (CK) and 30%, 50%, or 70% shade for 100 days. Growth, biomass, root traits, chlorophyll, invertase, soluble protein, stomatal characteristics, and epicuticular wax morphology were determined. ResultsShading significantly affected all measured traits. The 30% shade treatment produced the greatest seedling height, which increased by 118.18% compared with CK, and the highest chlorophyll content, which increased by 120.21%. Soluble protein content was slightly increased, whereas invertase activity decreased under moderate shading. Although total biomass decreased by 18.63%, root development remained relatively stable under 30% shade, with slight increases in total root length and average root diameter. Stomatal density was highest under this treatment, and the epicuticular wax structure remained relatively regular. In contrast, 70% shade markedly inhibited biomass accumulation and root development. ConclusionsModerate shading, particularly 30%, provided the most favorable light environment for Sorbus sibirica Dong Hong seedlings and is recommended for summer nursery cultivation in Northeast China.

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Huang, R., Gong, W., Li, X., Ji, S., Cui, T., Zhang, L.. 2026-07-27. Effects of different shading levels on growth, physiology and leaf surface micromorphology of Sorbus sibirica "Dong Hong" seedlings. https://doi.org/10.64898/2026.07.25.740310

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