Multi-site phenotypic characterisation of Kisumu colonies for comparison of insecticide susceptibility between testing centres

Harvey, G. F.; Praulins, G.; Akoupou, S.; Amlalo, G. K.; Anthony, S.; Azizi, S.; Bayili, K.; Bernard, E.; Corbel, V.; Dabire, R. K.; DIABATE, A.; Duchon, S.; Joannides, J.; Kisinza, W. N.; Koudou, B. B.; Mahande, A.; Manjurano, A.; Matowo, J.; Mawa, B.; Moore, S. J.; Mpelepele, A. B.; Mwaanga, G.; Mwingira, V.; N`dombidje, B.; N'Guessan, R.; Ngufor, C.; Ochomo, E.; Saidi, W. A.; Saili, K.; Simubali, L.; Stevenson, J. C.; Tchouakui, M.; Toguem, Y. F.; Walker, T.; Williams, J.; Wolie, R. Z.; Wondji, C. S.; Yalla, N. O.; Yirenkyi, A. O. D.; Zahouli, J. Z. B.; Mechan, F.; Wright, A.; Lees, R. S.

doi:10.64898/2026.07.21.739746

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bioRxiv · 10.64898/2026.07.21.739746

Multi-site phenotypic characterisation of Kisumu colonies for comparison of insecticide susceptibility between testing centres

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Abstract

Testing of insecticides and insecticide-based vector control tools relies on the use of bioassays whereby insects are exposed and mortality, or another relevant entomological endpoint, is measured. To be able to compare results between products and across time and space standard laboratory strains are used. Insecticide-susceptible strains are often used as comparators in studies involving insecticide-resistant populations. Kisumu is a long-established, widely-used insecticide-susceptible strain of Anopheles gambiae. In this study, 16 facilities across different countries exposed their Kisumu colonies aged 3-5 days to four insecticides (permethrin, alpha-cypermethrin, DDT and pirimiphos-methyl) at a range of concentrations in WHO tube assays to assess susceptibility. Insecticide-treated filter papers were made at the Liverpool School of Tropical Medicine and sent to testing centres, along with a standard protocol and testing conditions requirements, to standardise testing as much as possible. A first observation was that DDT results were affected by the use of Risella oil, the WHO-recommended carrier oil but immiscible in acetone, leading to uneven coating of papers and increased variability in bioassay results (40x variance between colonies). Bioassay data generated by the testing facilities suggest a wide range of susceptibility to different insecticides between colonies of Kisumu: LC50 values between colonies differed, though not significantly, by up to 4x for permethrin, 6x for pirimiphos-methyl, and 15x for alpha-cypermethrin. Some colonies are classified as insecticide resistant based on the WHO definition of <98% mortality on exposure to a discriminating concentration. The assumption that Kisumu is a standard susceptible strain, consistent between facilities is challenged, which has implications when comparing results between studies. We propose genotypic analysis of the colonies to identify signs of resistance markers indicative of contamination from resistant strains or wild mosquitoes, and generation of guidance to support the maintenance and monitoring of susceptible mosquito strains.

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Harvey, G. F., Praulins, G., Akoupou, S., Amlalo, G. K., Anthony, S., Azizi, S., Bayili, K., Bernard, E., Corbel, V., Dabire, R. K., DIABATE, A., Duchon, S., Joannides, J., Kisinza, W. N., Koudou, B. B., Mahande, A., Manjurano, A., Matowo, J., Mawa, B., Moore, S. J., Mpelepele, A. B., Mwaanga, G., Mwingira, V., N`dombidje, B., N'Guessan, R., Ngufor, C., Ochomo, E., Saidi, W. A., Saili, K., Simubali, L., Stevenson, J. C., Tchouakui, M., Toguem, Y. F., Walker, T., Williams, J., Wolie, R. Z., Wondji, C. S., Yalla, N. O., Yirenkyi, A. O. D., Zahouli, J. Z. B., Mechan, F., Wright, A., Lees, R. S.. 2026-07-22. Multi-site phenotypic characterisation of Kisumu colonies for comparison of insecticide susceptibility between testing centres. https://doi.org/10.64898/2026.07.21.739746

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