Domain-specific proteome remodeling defines mouse myelin aging

Freund, Y.; Schoof, M.; Moreno, N.; Kriegel, F.; Shin, H.; Waelchli, V.; Kaur, A.; Abraham, K.; Fogel, I.; Shi, S. M.; To, N.-S.; Guldner, I. H.; Cheung, T. H.; Yang, A. C.; Keller, A. H.; Wyss-Coray, T.; Iram, T.

doi:10.64898/2026.07.05.736479

bioRxiv · 10.64898/2026.07.05.736479

Domain-specific proteome remodeling defines mouse myelin aging

Freund, Y.·Schoof, M.·Moreno, N.·Kriegel, F.·Shin, H.·Waelchli, V.·Kaur, A.·Abraham, K.·Fogel, I.·Shi, S. M.·To, N.-S.·Guldner, I. H.·Cheung, T. H.·Yang, A. C.·Keller, A. H.·Wyss-Coray, T.·Iram, T.

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Abstract

Myelin, once regarded as a static insulating structure, is now recognized as a dynamic component of the nervous system, capable of remodeling in response to experience. Its breakdown is linked to cognitive decline and neurodegenerative diseases, often preceding neuronal dysfunction. While the myelin proteome has been studied, the age- and sex-related changes it undergoes remain poorly understood. In this study, we purified myelin proteins from young, middle-aged, and aged male and female mice. Using mass spectrometry-based proteomics, we identified 4,095 unique proteins in males and 3,931 in females, with roughly 30% showing significant age-related changes in both sexes. We find an age-related increase in compact myelin structural proteins, such as MBP, MOBP, and CLDN11, and a selective vulnerability in non-compact myelin cytoskeletal proteins, such as SEPTIN2, SEPTIN8, and OPALIN. Notably, disease-associated proteins previously characterized in single-cell transcriptomics appear at the protein level in aged myelin. To firmly distinguish between proteins derived from oligodendrocytes and other cell types in vivo we labeled nascent oligodendrocyte proteins with bio-orthogonal non-canonical amino acid tagging (BONCAT). We discovered a dramatic age-related increase in lysosomal and vesicle-associated proteins, while translation and synaptic proteins decrease in myelin with age in both sexes. This dataset highlights molecular mechanisms underlying the loss of myelin integrity and function with age and provides a novel tool for studying oligodendrocyte-derived nascent proteins in vivo.

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Freund, Y., Schoof, M., Moreno, N., Kriegel, F., Shin, H., Waelchli, V., Kaur, A., Abraham, K., Fogel, I., Shi, S. M., To, N.-S., Guldner, I. H., Cheung, T. H., Yang, A. C., Keller, A. H., Wyss-Coray, T., Iram, T.. 2026-07-09. Domain-specific proteome remodeling defines mouse myelin aging. https://doi.org/10.64898/2026.07.05.736479

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