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bioRxiv · 10.64898/2026.07.03.736325

IgA plasma cells co-secrete monomeric and dimeric IgA

thomas, J.·Eyer, K.·Wittner, J.·Rollenske, T.·Roth, E.·Xiang, W.·Schuh, W.·Jaeck, H.-M.·Mielenz, D.·Schulz, S.
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Abstract

Dimeric immunoglobulin A (dIgA) is generated from IgA monomers (mIgA) via JCHAIN-dependent polymerization. DIgA is transported across epithelial barriers by the poly Ig receptor (PIGR) and confers mucosal protection, while serum contains substantial amounts of IgA monomers. Distinct plasma cell subsets have been proposed to produce either monomeric or dimeric IgA, with bone marrow plasma cells as a primary source of mIgA. Here, we addressed whether IgA plasma cell populations segregate based on mIgA or dIgA production. Flow cytometric analysis of antibody-secreting cells from bone marrow, lymphoid and mucosal tissues revealed universal intracellular JCHAIN expression across isotypes and failed to identify a discrete JCHAIN-negative IgA plasma cell population. To detect polymeric IgA, we generated a recombinant soluble PIGR that selectively bound JCHAIN-containing dIgA in Western blot, ELISA, and flow cytometry. Soluble PIGR binding was detected in all IgA plasma cells irrespective of tissue origin, arguing against a dedicated mIgA-producing plasma cell subset incapable of dIgA formation. Ex vivo cultures and single-cell DropMap secretion assays demonstrated that bone marrow and lamina propria IgA antibody-secreting cells co-secrete mIgA and dIgA. These findings suggest that dIgA assembly and secretion are general properties of IgA plasma cells and disfavor a dedicated mIgA-producing population. HighlightsO_LIAll plasma cells express JCHAIN protein C_LIO_LIRecombinant poly Ig receptor detects dimeric IgA in all IgA plasma cells C_LIO_LIBone marrow plasma cells secrete dimeric IgA C_LIO_LIIgA plasma cells co-secrete mono- and dimeric IgA C_LI

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thomas, J., Eyer, K., Wittner, J., Rollenske, T., Roth, E., Xiang, W., Schuh, W., Jaeck, H.-M., Mielenz, D., Schulz, S.. 2026-07-08. IgA plasma cells co-secrete monomeric and dimeric IgA. https://doi.org/10.64898/2026.07.03.736325

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