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bioRxiv · 10.64898/2026.07.01.735572

Cell Penetrating Thyclotides Facilitate Efficient Delivery of Bioactive Peptides into Cells

Ayaz, G.·Zheng, H.·Amarasekara, H.·Clausse, V.·Tran, A. D.·Livak, F.·Kruhlak, M.·Appella, D.
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Abstract

Cell penetrating thyclotides (CPTs) are synthetic molecules that promote highly efficient cellular uptake and endosomal escape of bioactive peptides. While peptides are valuable as medicinal agents, their translation to therapies is often limited by their inability to cross cell membranes. CPTs have a unique combination of chiral tetrahydrofurans and polar sidechains within a molecular scaffold that can be optimized to efficiently deliver peptide cargo into cells. The cellular uptake and endosomal escape of two peptides with anticancer biological activities but low bioavailabilities were remarkably improved after conjugation to a CPT. Using CPTs to overcome barriers to cellular uptake represents a new direction for the intracellular delivery of bioactive molecules, and will accelerate drug development for new medical therapies.

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Ayaz, G., Zheng, H., Amarasekara, H., Clausse, V., Tran, A. D., Livak, F., Kruhlak, M., Appella, D.. 2026-07-02. Cell Penetrating Thyclotides Facilitate Efficient Delivery of Bioactive Peptides into Cells. https://doi.org/10.64898/2026.07.01.735572

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