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bioRxiv · 10.64898/2026.06.27.733865

Simultaneous Functional Ultrasound, Intrinsic Optical Signal and Widefield Calcium Neuroimaging

Mirg, S.·Gaddale, P.·Kumar, A.·Samanta, K.·Saini, B.·Patil, S. P.·Vargas, A. A.·Laliwala, A.·Exner, A. A.·Wang, Y.·Sipe, G. O.·Kothapalli, S.-R.
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Abstract

Functional ultrasound (fUS) maps cerebral blood volume (CBV) but lacks molecular and neuronal specificity. By simultaneously integrating fUS with optical imaging, we show that fUS-derived CBV correlates with both optically measured hemoglobin and neuronal calcium activity in awake mice. We further derive hemodynamic response functions linking calcium activity to CBV during spontaneous and sensory-evoked activity. Application to a mouse glioblastoma model demonstrates utility for studying neurovascular dysfunction in complex neuropathologies.

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Mirg, S., Gaddale, P., Kumar, A., Samanta, K., Saini, B., Patil, S. P., Vargas, A. A., Laliwala, A., Exner, A. A., Wang, Y., Sipe, G. O., Kothapalli, S.-R.. 2026-07-02. Simultaneous Functional Ultrasound, Intrinsic Optical Signal and Widefield Calcium Neuroimaging. https://doi.org/10.64898/2026.06.27.733865

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