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bioRxiv · 10.64898/2026.06.26.734836

Sequential infection reprograms the immune landscape to shape future responses

Abstract

Mouse models have been instrumental in defining immune mechanisms but often fail to capture the complexity of human immunity, limiting clinical translation. A major limitation is the immunological immaturity of specific pathogen-free (SPF) mice relative to pathogen-experienced adult humans. Here, we use a sequential infection (SI) model that recapitulates cumulative pathogen exposure and define its impact on immune composition and function. Beyond the previously reported expansion of memory T cells, SI induced durable, system-wide remodeling across lymphoid and non-lymphoid tissues, reshaping innate and adaptive immune populations, tissue-resident immunity, and hematopoietic output. Single-cell transcriptomic analyses revealed inflammatory imprinting of naive CD4 and CD8 T cells, whereas memory T cells acquired enhanced effector programs coupled with reduced biosynthetic activity, transcriptional states that more closely resemble those of pathogen-experienced adult humans. Functionally, SI mice recapitulated the human response to anti-CD28 super-agonist and exhibited altered magnitude and differentiation of acute and chronic antiviral T cell responses, demonstrating that cumulative pathogen exposure reshapes both existing immunity and the generation of future immune responses. Thus, cumulative pathogen exposure coordinately remodels hematopoiesis and naive and memory lymphocyte states, establishing a durable inflammation-experienced immune landscape that reshapes both immune memory and future immune responses, with broad implications for the translational fidelity of preclinical mouse models.

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Guo, M., Bouzaher, Y., Abd Rabbo, D., Quevedo, R., Elsaesser, H., Xu, W., Liu, M., Izzati, F., Ciudad, T., Bianca, M., Liu, K., Oliveira, J., Mortha, A., Edgar, L. J., McGaha, T. L., Reese, T. A., Brooks, D. G.. 2026-07-01. Sequential infection reprograms the immune landscape to shape future responses. https://doi.org/10.64898/2026.06.26.734836

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