Antifungal resistance mechanisms and nosocomial transmission of Nakaseomyces glabratus: genomic investigation and observational study in Melbourne, Australia

Gador-Whyte, A.; Seemann, T.; Judd, L. M.; Horan, K. A.; Lacey, J. A.; Traven, A.; Daniel, D.; Guerillot, R.; Giulieri, S.; Vogrin, S.; Aguilera, M. D.; Leroi, M.; Reynolds, G.; Howden, B. P.; Sherry, N. L.; Kwong, J. C.

doi:10.64898/2026.06.22.733717

bioRxiv · 10.64898/2026.06.22.733717

Antifungal resistance mechanisms and nosocomial transmission of Nakaseomyces glabratus: genomic investigation and observational study in Melbourne, Australia

Gador-Whyte, A.·Seemann, T.·Judd, L. M.·Horan, K. A.·Lacey, J. A.·Traven, A.·Daniel, D.·Guerillot, R.·Giulieri, S.·Vogrin, S.·Aguilera, M. D.·Leroi, M.·Reynolds, G.·Howden, B. P.·Sherry, N. L.·Kwong, J. C.

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Abstract

Nakaseomyces glabratus (Candida glabrata) is a WHO high-priority fungal pathogen associated with fungal antimicrobial resistance (fAMR). Given nosocomial transmission occurs sporadically, resistant strains could be transmitted, a concern for critically ill patients. We conducted a genomic investigation and retrospective observational study of N. glabratus to identify any nosocomial transmission of fAMR and understand resistance mechanisms and clinical and demiological factors among patients at a quaternary hospital in Melbourne, Australia. We selected stored N. glabratus with and without fAMR associated with similar patient clinical characteristics and performed whole genome sequencing. Clinical and epidemiological data were extracted from medical records. Phylogenetic, mutational, copy-number variation (CNV) and mitochondrial genomic analyses were performed, with a focus on the fAMR gene PDR1. Of 54 isolates collected over seven years, 20 (37%) were fluconazole-resistant and four (7%) had elevated flucytosine minimum inhibitory concentrations (MICs) (range 2-32 g/ml). There were no significant clinical differences between patients with and without fluconazole resistance. Most (55%) fluconazole-resistant isolates carried PDR1 mutations. Resistance was distributed throughout the phylogeny suggesting predominantly independent acquisition. However, a cluster of four resistant isolates with the same PDR1 mutation suggested nosocomial transmission. One probable ERG11 gene duplication, and two petite variants with apparent mitochondrial genomic deletions, were seen in association with fluconazole resistance. In this study, we identified a small probable nosocomial fAMR transmission cluster, and novel variants in PDR1, ERG11 and FCY2 associated with fAMR phenotypes. Future study should confirm functional impacts and systematically investigate for nosocomial transmission of resistance, including colonisation states.

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Gador-Whyte, A., Seemann, T., Judd, L. M., Horan, K. A., Lacey, J. A., Traven, A., Daniel, D., Guerillot, R., Giulieri, S., Vogrin, S., Aguilera, M. D., Leroi, M., Reynolds, G., Howden, B. P., Sherry, N. L., Kwong, J. C.. 2026-06-25. Antifungal resistance mechanisms and nosocomial transmission of Nakaseomyces glabratus: genomic investigation and observational study in Melbourne, Australia. https://doi.org/10.64898/2026.06.22.733717

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