Generation of three induced pluripotent stem cell lines from an immune checkpoint inhibitor-induced myocarditis patient and controls

Sun, Y.; Vitale, M.; Hnatiuk, A.; Wagner, N.; Sun, S.; Yang, X.; Liu, L.; Khatua, S.; Sundar, H.; Chou, H.; Huang, Y.; Waliany, S.; Zhuge, Y.; Witteles, R.; Mercola, M.; Wu, J.; Zhu, H.

doi:10.64898/2026.06.17.730743

bioRxiv · 10.64898/2026.06.17.730743

Generation of three induced pluripotent stem cell lines from an immune checkpoint inhibitor-induced myocarditis patient and controls

Sun, Y.·Vitale, M.·Hnatiuk, A.·Wagner, N.·Sun, S.·Yang, X.·Liu, L.·Khatua, S.·Sundar, H.·Chou, H.·Huang, Y.·Waliany, S.·Zhuge, Y.·Witteles, R.·Mercola, M.·Wu, J.·Zhu, H.

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Abstract

Immune checkpoint inhibitor-associated myocarditis (ICIM) is an uncommon but potentially fatal inflammatory heart disease triggered by cancer immunotherapy, with up to 40% mortality. The underlying mechanisms are still elusive, partly due to the lack of appropriate human disease models. Here, we report the generation of three induced pluripotent stem cell (iPSC) lines derived from an ICIM patient, an ICI-treated patient without myocarditis, and a healthy donor. These lines exhibit typical pluripotent stem cell morphology, express pluripotency markers, maintain normal karyotypes, and differentiate into derivatives of the three germ layers, providing a valuable platform for mechanistic studies and therapeutic discovery.

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Sun, Y., Vitale, M., Hnatiuk, A., Wagner, N., Sun, S., Yang, X., Liu, L., Khatua, S., Sundar, H., Chou, H., Huang, Y., Waliany, S., Zhuge, Y., Witteles, R., Mercola, M., Wu, J., Zhu, H.. 2026-06-22. Generation of three induced pluripotent stem cell lines from an immune checkpoint inhibitor-induced myocarditis patient and controls. https://doi.org/10.64898/2026.06.17.730743

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