Molecular and ecological determinants of effective reassortment in orthohantaviruses

Rivero, R.; Simons, D.; Damodaran, L.; Karegi, I.; Gurev, S.; Becker, D. J.; Warren, D. L.; Mueller, N. F.; Rasmussen, D. A.; Seifert, S. N.

doi:10.64898/2026.06.10.731004

bioRxiv · 10.64898/2026.06.10.731004

Molecular and ecological determinants of effective reassortment in orthohantaviruses

Rivero, R.·Simons, D.·Damodaran, L.·Karegi, I.·Gurev, S.·Becker, D. J.·Warren, D. L.·Mueller, N. F.·Rasmussen, D. A.·Seifert, S. N.

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Abstract

A central unsolved problem in RNA virus evolution is understanding why some viral reassortants establish and persist while others do not. To answer this question, we reconstructed reassortant histories across 553 viral genomes from seven orthohantavirus species between 1983 and 2024 using phylogenetic reconciliation and molecular dating. We found that the frequency of retained reassortants varied among orthohantaviruses. For example, reassortment ranged from absent in Andes virus to frequent in Dobrava-Belgrade, Sin Nombre, Seoul, Puumala, and Tula viruses, showing that effective reassortment is not a genus-wide constant. Our Bayesian hierarchical models identified local host overlap as the strongest ecological factor associated with viral reassortment, while cross-segment linkage and terminal RNA structure serve as a molecular filter. We found that the probability of reassortment establishment is highest when ecological opportunity is paired with molecular permissiveness, with their interaction term inferred as the strongest signal in our establishment models (posterior probability = 0.97). These results suggest that reassortment in orthohantaviruses is a sequentially filtered evolutionary process in which divergent lineages must first meet in a host through ecological overlap, exchange segments that are molecularly compatible, and do so within a lineage background permissive to establishment in the host population.

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Rivero, R., Simons, D., Damodaran, L., Karegi, I., Gurev, S., Becker, D. J., Warren, D. L., Mueller, N. F., Rasmussen, D. A., Seifert, S. N.. 2026-06-12. Molecular and ecological determinants of effective reassortment in orthohantaviruses. https://doi.org/10.64898/2026.06.10.731004

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