Diversifying the Northern Neotropics: Phylogenomics and Evolutionary History of the Early-Diverging Herichthyine Cichlids Thorichthys and Trichromis

Abstract

Among Neotropical cichlids the tribe Heroini exhibits substantial ecological diversity and is one of the dominant fish groups across northern Neotropical riverscapes. The majority of studies on heroine cichlids have focused on macroevolutionary patterns but the role of geological and ecological factors shaping lineage diversification within the tribe remains poorly understood. Here we used ultraconserved elements (UCEs) to infer a taxonomically complete and geographically comprehensive phylogenomic framework of the early-diverging herichthyine sister genera Thorichthys and Trichromis and to comparatively investigate their evolutionary and biogeographic histories. All our phylogenomic hypotheses support the monophyly of both genera and two species subgroups within Thorichthys. Our results provide evidence of a) unrecognized diversity within Trichromis salvini, b) uncertainty in species boundaries in Thorichthys, and c) the first report of ghost introgression in fishes of the northern Neotropics. Additionally, our results demonstrate the importance of the Papaloapan and Coatzacoalcos watersheds for fish diversification in the region. Finally, we show patterns consistent with ecological divergence during the evolution of this group, particularly among sympatric species. Altogether, these patterns suggest that lineage diversification in northern Neotropical cichlids has been driven by the interaction of geological restructuring, climatic and sea-level oscillations, and heterogeneous ecological pressures.