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bioRxiv · 10.64898/2026.06.03.729976

Architectural fragility of gene regulatory networks underlies hematopoietic stem cell aging

Abstract

Hematopoietic stem and progenitor cell (HSPC) aging contributes to immune dysfunction and age-associated disease, but its regulatory mechanisms remain unclear. Here, we present the largest single-cell multiome atlas of human circulating HSPCs to date, with >380,000 paired RNA and ATAC profiles across 77 donors. Beyond recapitulating established hallmarks of HSPC aging, we reconstructed a high-resolution gene regulatory network and identified a global rewiring in which stress-response and myeloid transcription factor (TF) programs expand, while self-renewal and lymphoid lineage-defining circuitry collapses. We associate these phenotypes with increased cis-regulatory entropy, including elevated transcriptomic noise, weakened peak-to-gene coupling, and chromatin peak broadening. This rewiring is selective, where TFs with GC-rich, promoter-proximal architectures are preserved or amplified, and complex, distal enhancer-dependent identity networks are eroded. Thus, these findings suggest that progressive entropic destabilization of gene regulatory architecture simultaneously drives stress hyperactivation, myeloid bias, and identity loss in aging HSPCs.

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BibTeXRIS

Stevens, H. P., Yucel, A. D., Gould, R. A., Cai, G., Gladyshev, V. N., Plesa, A. M., Church, G. M.. 2026-06-04. Architectural fragility of gene regulatory networks underlies hematopoietic stem cell aging. https://doi.org/10.64898/2026.06.03.729976

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