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bioRxiv · 10.64898/2026.06.03.728752

Airspace miR-146a levels in ventilated patients decrease with age and correlate with mortality

Abstract

The acute respiratory distress syndrome is a heterogenous syndrome characterized by the rapid development of respiratory failure. Nearly 40% of patients who develop ARDS will die, and there is growing interest in identification of biomarkers to identify patients at risk of death and/or inform treatment decisions. Most prior work on biomarkers in ARDS has focused on the plasma compartment, but there is concern that circulating biomarkers may not reflect alveolar pathobiology. The anti-inflammatory microRNA-146a has been shown to be upregulated in inflammatory cells in human bronchoalveolar lavage fluid, but it is not known if these levels correspond with outcomes. We measured miR-146a expression by digital droplet PCR in human biospecimens from four different cohorts of patients with respiratory failure requiring mechanical ventilation - two plasma cohorts, one bronchoalveolar lavage cohort, and one heat moisture exchange (HME) filter fluid cohort. We found that miR-146a was detectible in plasma, bronchoalveolar lavage fluid, and HME fluid. However, only when measured in the alveolar space, was miR-146a expression significantly lower in older adults and those who died. It did not correlate with outcomes when measured in plasma. To our knowledge, this is the first report that nucleotides can be measured in HME fluid and builds upon expanding literature that circulating biomarkers may not reflect complex biology of the alveolar microenvironment during ARDS.

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BibTeXRIS

Bentley, I. D., Kapoor, A., Gulick, N., Langenecker, M., Leuenberger, L. A., Morrell, E. D., Bednash, J. S., Mikacenic, C., Shaver, C. M., Englert, J. A.. 2026-06-08. Airspace miR-146a levels in ventilated patients decrease with age and correlate with mortality. https://doi.org/10.64898/2026.06.03.728752

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