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bioRxiv · 10.64898/2026.06.01.729412

Dorsal striatal circuit mechanisms contributing to astrocyte modulation of alcohol-related behaviors

Ardinger, C.·Kalelkar, A.·Madden, M.·Gunda, A.·Patel, A.·Xanthos, G.·Mahboob, M.·Khawaja, A.·Collie-Beard, N.·Bocarsly, M.·Huda, R.
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Abstract

BackgroundThe dorsolateral striatum (DLS) is a key site for coordinating the alcohol-induced stimulant response, a behavioral marker predictive of future alcohol use disorder. Although ethanol (EtOH) affects all brain cells, little is known about the contribution of non-neuronal DLS cell types to EtOH-induced stimulation. MethodsWe used ex vivo two photon calcium imaging, in vivo fiber photometry of astrocyte and neuronal GCaMP, and astrocyte-specific manipulations in mice to determine DLS astrocyte contributions to EtOH-induced stimulation and voluntary EtOH drinking behavior. Using fiber photometry of GRAB-ACh sensors and cell-type specific chemogenetics, we also assessed the role of cholinergic signaling in observed astrocyte EtOH effects. ResultsAs expected, intraperitoneal EtOH injections (0.5-2g/kg) evoked a stimulant response, evidenced by increased locomotion compared to saline. In parallel, EtOH dose-dependently decreased astrocyte calcium activity but had minimal effects on direct and indirect pathway neuronal activity. Mimicking this reduction with astrocyte-specific expression of CalEX, a calcium extruding pump, facilitated EtOH stimulation compared to mice expressing a control fluorophore. Hence, EtOH-induced suppression of DLS astrocyte activity contributes to stimulation. Astrocyte calcium signaling is a well-known target of neuromodulation. Fiber photometry recordings of extracellular acetylcholine (ACh) levels via GRAB-ACh imaging showed inhibition of ACh release by acute EtOH. We virally expressed the excitatory chemogenetic actuator hM3Dq in striatal cholinergic interneurons to assess whether artificially increasing ACh release blocks EtOH-induced inhibition of astrocytic calcium activity. Despite facilitating ACh release, this manipulation did not impact astrocyte calcium activity under control (saline) or EtOH conditions. Together, this work identifies DLS astrocytes as key contributors to EtOH-induced stimulation and highlights the importance of considering astrocyte-neuron interactions in evaluating alcohol effects.

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Ardinger, C., Kalelkar, A., Madden, M., Gunda, A., Patel, A., Xanthos, G., Mahboob, M., Khawaja, A., Collie-Beard, N., Bocarsly, M., Huda, R.. 2026-06-04. Dorsal striatal circuit mechanisms contributing to astrocyte modulation of alcohol-related behaviors. https://doi.org/10.64898/2026.06.01.729412

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