Enterovirus-driven interferon signaling induces epithelial TG2 via JAK-STAT: Implications for the onset of celiac disease

Hien Le, H.; Rakkolainen, V.; Davidsson, R.; Dotsenko, V.; Martin Diaz, L.; Sioofy Khojine, A.; Virtanen, A.; Laiho, J. E.; Khosla, C.; Silvennoinen, O.; Hyoty, H.; Viiri, K.

doi:10.64898/2026.05.26.727875

bioRxiv · 10.64898/2026.05.26.727875

Enterovirus-driven interferon signaling induces epithelial TG2 via JAK-STAT: Implications for the onset of celiac disease

Hien Le, H.·Rakkolainen, V.·Davidsson, R.·Dotsenko, V.·Martin Diaz, L.·Sioofy Khojine, A.·Virtanen, A.·Laiho, J. E.·Khosla, C.·Silvennoinen, O.·Hyoty, H.·Viiri, K.

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Abstract

Background & AimsCeliac disease (CeD) is an autoimmune disorder triggered by dietary gluten in genetically predisposed individuals, but environmental factors contributing to disease onset remain incompletely defined. Epidemiological studies implicate enterovirus infections as potential triggers. Here, we investigated the epithelial-intrinsic mechanisms by which coxsackievirus B1 (CVB1) infection may prime the intestine for CeD. MethodsHuman intestinal organoids were infected with CVB1 and analyzed using single-cell RNA sequencing to resolve lineage-specific responses. Interferon signaling and transglutaminase 2 (TG2) regulation were interrogated using type I interferon stimulation and pharmacologic JAK inhibition. ResultsCVB1 infection induced a robust epithelial antiviral program dominated by type I interferon signaling. This response was accompanied by marked upregulation of TG2 expression and enzymatic activity. Single-cell analysis localized TG2 induction to immature goblet-lineage cells, which exhibited strong interferon-stimulated gene activation and epithelial stress signatures. Mechanistically, IFN-/{beta} stimulation was sufficient to induce TG2 via JAK-STAT signaling, while JAK inhibition effectively suppressed both TG2 expression and activity. In parallel, CVB1 infection triggered coordinated mucin remodeling, including induction of MUC5AC, indicating interferon-linked epithelial reprogramming. Notably, these effects occurred independently of immune cell involvement, highlighting a cell-intrinsic pathway. ConclusionOur findings identify a direct epithelial mechanism linking enterovirus infection to TG2 activation via interferon-driven JAK-STAT signaling. This pathway provides a mechanistic bridge between viral infection and gluten peptide modification, a critical step in the onset of CeD. The reversibility of TG2 induction by JAK inhibition suggests a potential strategy to prevent virus-mediated priming of celiac disease.

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Hien Le, H., Rakkolainen, V., Davidsson, R., Dotsenko, V., Martin Diaz, L., Sioofy Khojine, A., Virtanen, A., Laiho, J. E., Khosla, C., Silvennoinen, O., Hyoty, H., Viiri, K.. 2026-05-29. Enterovirus-driven interferon signaling induces epithelial TG2 via JAK-STAT: Implications for the onset of celiac disease. https://doi.org/10.64898/2026.05.26.727875

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