Dual histone methylation reader ZCWPW2 links histone methylation to initiation of meiotic recombination

Yuan, S.; Wang, S.; Bao, Z.; Wang, Z.; Shangguan, K.; Yan, Y.; Shi, Y.; Feng, X.; Huang, C.; Fang, M.; Zhao, H.; Zhao, S.; Liu, H.; Chen, Z.-J.; Huang, T.

doi:10.64898/2026.05.22.727067

bioRxiv · 10.64898/2026.05.22.727067

Dual histone methylation reader ZCWPW2 links histone methylation to initiation of meiotic recombination

Yuan, S.·Wang, S.·Bao, Z.·Wang, Z.·Shangguan, K.·Yan, Y.·Shi, Y.·Feng, X.·Huang, C.·Fang, M.·Zhao, H.·Zhao, S.·Liu, H.·Chen, Z.-J.·Huang, T.

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Abstract

Meiotic homologous recombination initiates with the formation of programmed DNA double-strand breaks (DSBs) by complexes comprising SPO11 and accessory proteins at discrete sites called recombination hotspots. In mammals, PRDM9-dependent H3K4me3 and H3K36me3 define recombination hotspots, but how these epigenetic characteristics determine the physiological DSB formation remains unknown. Here we show that dual histone methylation reader ZCWPW2 can recognize H3K4me3 and H3K36me3 marks in testis. The binding activity of ZCWPW2 to histone methylation is dependent on PRDM9 function. Moreover, we find the epigenetic writer-reader axis PRDM9-ZCWPW2 is essential for DSB formation at meiotic recombination hotspots, which may be partly explained by the finding that ZCWPW2 can physically interact with HORMAD1, IHO1, MEI4, and REC114. Finally, the absence of ZCWPW2 leads to disrupted chromosomal synapsis and recombination, thereby obstructing meiotic progression. Taken together, our findings provide new insights into how histone modifications and their associated regulatory proteins collectively regulate meiotic homologous recombination initiation.

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Yuan, S., Wang, S., Bao, Z., Wang, Z., Shangguan, K., Yan, Y., Shi, Y., Feng, X., Huang, C., Fang, M., Zhao, H., Zhao, S., Liu, H., Chen, Z.-J., Huang, T.. 2026-05-26. Dual histone methylation reader ZCWPW2 links histone methylation to initiation of meiotic recombination. https://doi.org/10.64898/2026.05.22.727067

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