SSB1 links stress granule regulation to cellular stress responses and renal ischemia-reperfusion injury

Palinkas, J.; Jezso, B.; Nagy-Kanta, E.; Nemeth, R.; Aman, U. A.; Takacs, G.; Szikriszt, B.; Hosszu, A. T.; Ecsedi, P.; Szakacs, G.; Szuts, D.; Fekete, A.; Kovacs, M.

doi:10.64898/2026.05.22.726875

bioRxiv · 10.64898/2026.05.22.726875

SSB1 links stress granule regulation to cellular stress responses and renal ischemia-reperfusion injury

Palinkas, J.·Jezso, B.·Nagy-Kanta, E.·Nemeth, R.·Aman, U. A.·Takacs, G.·Szikriszt, B.·Hosszu, A. T.·Ecsedi, P.·Szakacs, G.·Szuts, D.·Fekete, A.·Kovacs, M.

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Abstract

Mammalian single-stranded DNA binding protein 1 (SSB1) has been established as an essential component of genome stability in both human cells and mice. Moreover, SSB1 was recently implicated in cytoplasmic stress response by its involvement in Ras GTPase-activating protein-binding protein 1 (G3BP1)-containing cytoplasmic stress granules (SGs) upon various forms of stress. Here, we generated and analyzed human cellular knockout and rodent ischemia-reperfusion (I/R) models to define SSB1s roles in cytoplasmic stress response. Analysis of wild-type as well as SSB1 and G3BP1 knockout human retinal pigment epithelial (RPE-1) cells shows stress-specific incorporation of SSB1 into SGs and a negative regulator role for SSB1 in SG dynamics under sublethal stress conditions. We find that SSB1 knockout measurably increases cellular sensitivity to oxidative stress but does not alter cell proliferation following mild acute stress. Moreover, we detect SSB1 efflux from the nucleus upon stress that is dependent upon the presence of G3BP1 in a stress-specific manner. In addition, using mouse and rat models we observe significant upregulation and robust cytoplasmic granulation of SSB1 upon renal ischemia-reperfusion stress, establishing SSB1s involvement in complex organismal stress response in vivo. Together, our data demonstrate active involvement of SSB1 in cytoplasmic response to cellular stress and acute kidney injury, with implications for targeting stress response functions in cancerous versus non-cancerous contexts. HIGHLIGHTSO_LISSB1 is incorporated into cytoplasmic stress granules and negatively regulates stress granule assembly under sublethal stress conditions C_LIO_LISSB1 shows stress- and G3BP1-dependent nuclear efflux C_LIO_LISSB1 is upregulated and undergoes apical granulation in renal epithelial cells during renal ischemia-reperfusion injury C_LI

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Palinkas, J., Jezso, B., Nagy-Kanta, E., Nemeth, R., Aman, U. A., Takacs, G., Szikriszt, B., Hosszu, A. T., Ecsedi, P., Szakacs, G., Szuts, D., Fekete, A., Kovacs, M.. 2026-05-26. SSB1 links stress granule regulation to cellular stress responses and renal ischemia-reperfusion injury. https://doi.org/10.64898/2026.05.22.726875

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