A Bioprinted Head and Neck Cancer Organoid-Based Platform for Evaluating Multimodal Therapies

Lin, L.; Bommakanti, K. K.; Wooten, C.; Gonzalez, A. E.; Alhiyari, Y.; Levi, J.; Wang, B.; Sannajust, A.; Evans, L. K.; Tebon, P.; St. John, M. A.; Soragni, A.

doi:10.64898/2026.05.20.726741

bioRxiv · 10.64898/2026.05.20.726741

A Bioprinted Head and Neck Cancer Organoid-Based Platform for Evaluating Multimodal Therapies

Lin, L.·Bommakanti, K. K.·Wooten, C.·Gonzalez, A. E.·Alhiyari, Y.·Levi, J.·Wang, B.·Sannajust, A.·Evans, L. K.·Tebon, P.·St. John, M. A.·Soragni, A.

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Abstract

Treatment of advanced head and neck squamous cell carcinoma (HNSCC) often involves radiotherapy combined with chemotherapy, targeted therapy, or immunotherapy. However, due to its anatomical and molecular heterogeneity, identifying the most effective treatment for each patient remains a major clinical challenge. To address this need, we developed a high-throughput organoid-based drug screening platform that uses patient-derived organoids to assess candidate treatment regimens. We validated the platform by establishing bioprinted 3D organoids of human HNSCC cell lines and exposing them to X-ray radiation in combination with various small-molecule drugs and biologics. We quantified viability using ATP release assays and assessed extracellular matrix (ECM) invasion with a machine learning-based brightfield image analysis pipeline. Proof-of-concept experiments with HPV-negative HNSCC lines (HN30 and HN31, established from primary and metastatic disease from the same patient) and HPV-positive HNSCC cells (SCC154) revealed different therapy agents that can radiosensitize each cell line. Image analysis showed that copanlisib, afatinib, and ibrutinib could limit ECM invasion of HN31, while the AKT inhibitor ipatasertib promotes invasion of HN30 cells, consistent with previous studies. Application of the platform to patient-derived HPV+ oropharyngeal tumor organoids showed that they shared sensitivity to several agents while also exhibiting differences against certain therapies. Cetuximab, sorafenib, and nedisertib significantly radiosensitized organoids from two clinical samples. This work demonstrates the feasibility of performing sensitivity screening by integrating bioprinting, conventional viability assays, and advanced image analysis techniques. This platform has the potential to enable a personalized therapeutic pipeline for patients with advanced HNSCC, optimizing responses to radiotherapy and targeted agents to improve clinical outcomes while avoiding modulators that may promote tumor invasion.

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Lin, L., Bommakanti, K. K., Wooten, C., Gonzalez, A. E., Alhiyari, Y., Levi, J., Wang, B., Sannajust, A., Evans, L. K., Tebon, P., St. John, M. A., Soragni, A.. 2026-05-21. A Bioprinted Head and Neck Cancer Organoid-Based Platform for Evaluating Multimodal Therapies. https://doi.org/10.64898/2026.05.20.726741

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