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bioRxiv · 10.64898/2026.05.20.726515

Spatial transcriptomics identifies the Pseudomonas aeruginosa major outer membrane protein OprF as a critical factor in blinding corneal infections

Abbondante, S.·Zhu, H.·Marshall, M. E.·Chumbler, N.·Negron, O.·Tyagi, A.·Rietsch, A.·Pearlman, E.·Gadjeva, M.
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Abstract

Pseudomonas aeruginosa is a globally recognized pathogen causing pulmonary, skin, and severe corneal infections (keratitis), with the potential to induce irreversible blindness if untreated. Spatial transcriptomic analysis of P. aeruginosa infected corneas identified elevated expression of the outer membrane proteins OprF and OprL and PA1414, which encodes the small RNA SicX in the corneal stroma compared with corneal epithelium. Comparative spatial transcriptomics analysis of corneas infected with an oprF transposon (TN) mutant showed reduced expression of the type III effector protein ExoT, which was absent in an oprF deficient mutant ({Delta}oprF) and in contrast to PA14, did not inhibit reactive oxygen species (ROS) production by neutrophils. Corneal infection with the {Delta}oprF mutant resulted in reduced corneal virulence and lower CFU compared to the parental PA14 strain. Collectively, our findings demonstrate a coordinated virulence program connecting OprF functionality with the release of ExoT and its ability to block ROS production and survive in infected corneas.

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Abbondante, S., Zhu, H., Marshall, M. E., Chumbler, N., Negron, O., Tyagi, A., Rietsch, A., Pearlman, E., Gadjeva, M.. 2026-05-20. Spatial transcriptomics identifies the Pseudomonas aeruginosa major outer membrane protein OprF as a critical factor in blinding corneal infections. https://doi.org/10.64898/2026.05.20.726515

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