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bioRxiv · 10.64898/2026.05.19.726119

DNA Double-decker Ring Scaffolded Nanodisc for Self-assembly of Membrane Protein into Lipid Bilayer

Aye, S. L.·Fadaei, F.·Gomibuchi, Y.·Suzuki, Y.·Prakash, P. S.·Chandrasekhar, S.·Yasunaga, T.·Schmidt, T.-L.·Sato, Y.
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Abstract

Membrane models of scaffolded discoidal lipid bilayers called nanodiscs have proven to be a valuable tool for the study of membrane proteins in a native environment. DNA-scaffolded membrane model has emerged as an alternative tool for membrane protein studies. Taking advantage of the designability of DNA nanostructure, we created a double-decker double-stranded DNA ring (DDring) to self-assemble DNA-based nanodiscs (DNA-ND). The DDring is 17 nm wide and 4 nm high, and equipped with 28 alkyl chains on the inside that can interact with each hydrophobic leaflet of the lipid bilayer. We further demonstrate the functionality of DNA-ND membrane model with the assembly of membrane proteins. DDrings are suited to neutral or cationic charged phospholipids and detergents. This study provides more insights into the potential use of DNA- assisted nanodiscs for membrane protein characterization.

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Aye, S. L., Fadaei, F., Gomibuchi, Y., Suzuki, Y., Prakash, P. S., Chandrasekhar, S., Yasunaga, T., Schmidt, T.-L., Sato, Y.. 2026-05-21. DNA Double-decker Ring Scaffolded Nanodisc for Self-assembly of Membrane Protein into Lipid Bilayer. https://doi.org/10.64898/2026.05.19.726119

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